We narrowed to 2,296 results for: pET28

1. proISG15 C-term. domain (79-165)

   Plasmid

   #110763

   Purpose

   Bacterial expression of proISG15 C-term. domain

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. His6-SMT3-SopB(C460S)

   Plasmid

   #183677

   Purpose

   Recombinant Salmonella Typhimurium SopB (catalytically inactive)

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
   

   Use

   Tags

   His6-S. cerevisiae SMT3(2-101)

   Expression

   Bacterial

   Mutation

   C460S

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Met1 diUb (1-152, R42W)

   Plasmid

   #110767

   Purpose

   Bacterial expression of Met1 diUb

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   UBB (UBB Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   R42W

   Promoter

   T7

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MEK1C222

   Plasmid

   #164638

   Purpose

   Bacterial expression plasmid for His6-MEK1C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   G(-19)F/S222C/C277S/C376S

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MEK1C218

   Plasmid

   #164639

   Purpose

   Bacterial expression plasmid for His6-MEK1C218

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   G(-19)F/S218C/C277S/C376S

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. His6-EGFP-cPHx1

   Plasmid

   #183674

   Purpose

   PtdIns(3,4)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLEKHA1 (PLEKHA1 Human)
   

   Use

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 169-329

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. MEK1C218C222

   Plasmid

   #164640

   Purpose

   Bacterial expression plasmid for His6-MEK1C218C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   G(-19)F/S218C/S222C/C277S/C376S

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mature ISG15 C-term. Domain (79-165, W123A)

   Plasmid

   #110765

   Purpose

   Bacterial expression of mutant proISG15 C-term. domain

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   proISG15 C-term. domain (79-165, W123A) (ISG15 Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   W123A

   Promoter

   T7

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDL52 (clone45)

   Plasmid

   #188578

   Purpose

   High-yield production of coenzyme F420 in E. coli

   Depositor

   Gerald Lackner

   Article

   Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

   Inserts

   ribA
   ribD
   yigB
   fbiC
   cofC
   cofD
   cofI
   cofE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimization, synthetic ribosome binding si…

   Promoter

   T7 variants

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDL52 (clone76)

    Plasmid

    #188579

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSumo24P7vB4

    Plasmid

    #113073

    Purpose

    Plasmid for highly efficient expression of engineered IL24 with binding affinity to cognate receptors

    Depositor

    Bohdan Schneider

    Article

    Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

    Insert

    Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 SUMO part (Brachypodium distachyon), Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

    Promoter

    T7

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSumo24P7

    Plasmid

    #113072

    Purpose

    Plasmid for highly efficient expression of engineered IL24 with mutated binding sites

    Depositor

    Bohdan Schneider

    Article

    Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

    Insert

    Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 SUMO part (Brachypodium distachyon), Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

    Promoter

    T7

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Δ1-60MEK1C222

    Plasmid

    #164641

    Purpose

    Bacterial expression plasmid for His6-ΔMEK1C222

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    S222C/C277S/C376S; residues 1-60 at N-terminus we…

    Promoter

    T7

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Δ1-60MEK1C218

    Plasmid

    #164643

    Purpose

    Bacterial expression plasmid for His6-ΔMEK1C218

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    S218C/C277S/C376S; residues 1-60 at N-terminus we…

    Promoter

    T7

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Δ1-60MEK1C218C222

    Plasmid

    #164645

    Purpose

    Bacterial expression plasmid for His6-ΔMEK1C218C222

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    S218C/S222C/C277S/C376S; residues 1-60 at N-termi…

    Promoter

    T7

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNICBio2_WDR5:2-334

    Plasmid

    #211027

    Purpose

    bacterial expression of human WDR5 fragment A2 to C334. N-terminal 10X His tag with TEV cleavage and C-terminal biotin acceptor peptide tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    WDR5:A2-C334 (WDR5 Human)
    

    Use

    Tags

    MHHHHHHHHHHDLGTENLYFQS and SSKGGYGLNDIFEAQKIEWHE

    Expression

    Bacterial

    Mutation

    wild type

    Promoter

    T7

    Available since

    April 4, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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