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We narrowed to 5,538 results for: pcas

Showing: 2281 - 2300 of 5538 results

  1. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid
    #100597
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100597-plasmid-map-sequence-id-207349

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  3. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

    Plasmid
    #139995
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    HF1=N497A/R661A/Q695A/Q926A
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139995-plasmid-map-sequence-id-275425

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  4. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid
    #100596
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100596-plasmid-map-sequence-id-207339

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  8. pCAG-FLAG-puro-PTPIP51(1-150_175-470)

    Plasmid
    #170537
    Purpose
    Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection
    Depositor
    Byung Il Lee
    Article
    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:
    Insert
    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    Use
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    deleted amino acids 151-175
    Promoter
    CAG promoter
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only

  14. pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid
    #116955
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    116955-plasmid-map-sequence-id-226973

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Showing: 2281 - 2300 of 5538 results