178,119 results

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pCS6-YFP-SMASh

Plasmid

#68853

Purpose

Expresses SMASh tag fused to YFP at C terminus in mammalian cells

Depositor

Michael Lin

Publication

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YFP-SMASh

Tags

SMASh tag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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secreted BirA-Flag

Plasmid

#64395

Purpose

Flag-tagged biotinylation enzyme for co-expression with bio-proteins

Depositor

Gavin Wright

Publication

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BirA (birA E. coli)

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64218

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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2C::tdTomato Reporter

Plasmid

#40281

Depositor

Samuel Pfaff

Publication

Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

2C Promoter (2-cell stage promoter)

Tags

tdTomato

Expression

Mammalian

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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LeGO-iG2

Plasmid

#27341

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-eGFP

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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myc-hULK1

Plasmid

#31961

Depositor

Do-Hyung Kim

Publication

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ULK1 (ULK1 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pAc-Neo-OVA

Plasmid

#22533

Depositor

Michael Bevan

Publication

Moore et al Cell. 1988 Sep 9. 54(6):777-85.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Available since

Dec. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAL175

Plasmid

#22276

Purpose

Expresses YFP-PIF for red-light-induced binding to Phy

Depositor

Christopher Voigt

Publication

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

SV40

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGL2Basic-EcadK1

Plasmid

#19290

Depositor

Eric Fearon

Publication

Hajra et al Oncogene. 1999 Dec 2. 18(51):7274-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E-cadherin

Use

Luciferase

Expression

Mammalian

Mutation

E-cad promoter sequences from -108 to +125

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMIG-hOCT4

Plasmid

#17225

Depositor

George Daley

Publication

Park et al Nature. 2008 Jan 10. 451(7175):141-6.

Insert

POU class 5 homeobox 1 (POU5F1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pcDNA3-DN-hCUL4A-FLAG

Plasmid

#15821

Depositor

Wade Harper

Publication

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cullin4A (CUL4A Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N440

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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HA-CYLD-WT

Plasmid

#15506

Depositor

Stephen Elledge

Publication

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

CYLD (CYLD Human)

Tags

HA

Expression

Mammalian

Available since

Aug. 13, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV-SynA

Plasmid

#174860

Purpose

Expresses mouse syncytin A in mammalian cells

Depositor

Feng Zhang

Publication

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

syncytin A (Syna Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

Plasmid

#153004

Purpose

Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

Depositor

Alice Ting

Publication

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (N) (G78)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA puro

Plasmid

#104990

Purpose

This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

Plasmid

#103870

Purpose

High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.

Depositor

Feng Zhang

Publication

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

PspCas13b
huADAR2DD (ADARB1 Human)

Use

CRISPR

Tags

GSGGGGS and HIV NES

Expression

Mammalian

Mutation

Changed histidne 133 to alanine and histidine 105…

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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SETD2-GFP

Plasmid

#80653

Purpose

mammalian expression of SETD2-GFP

Depositor

Sérgio De Almeida

Publication

Carvalho et al Elife. 2014 May 6;3:e02482. doi: 10.7554

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SETD2 (SETD2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pIMAY

Plasmid

#68939

Purpose

E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

Depositor

Tim Foster

Publication

Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

Type

Empty backbone

Use

Staphylococcal allelic exchange vector

Available since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pLV_hEF1a_rtTA3

Plasmid

#61472

Purpose

3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

Depositor

Ron Weiss

Publication

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Insert

rtTA3

Use

Lentiviral

Promoter

hEF1a

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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