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Plasmid#216484PurposeUsed for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tagDepositorInsertADP-ribosylation factor like 16 (ARL16 Human)
UseTagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/hFGFR3-K650M-V5
Plasmid#214909Purposeexpression of the K650M mutant variant of human FGFR3, which is associated with Severe Achondroplasia with Developmental Delay and Acanthosis Nigricans (SADDAN)DepositorInsertFGFR3 (FGFR3 Human)
UseTagsV5/HisExpressionMammalianMutationK650M substitutionPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mGCC2-FLAG
Plasmid#214166PurposeTransient expression of GCC2DepositorInsertGCC2 (Gcc2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnTS-E1015A
Plasmid#213415PurposeVinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).DepositorInsertVcnTS-E1015A (VCL Chicken, Synthetic)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1015 to alanine (E…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnTS-E1021A
Plasmid#213416PurposeVinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).DepositorInsertVcnTS-E1021A (VCL Chicken, Synthetic)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1021 to alanine (E…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnCS-E1015A
Plasmid#213412PurposeVinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).DepositorInsertVcnCS-E1015A (VCL Chicken, Synthetic)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1015 to alanine (E…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnCS-E1021A
Plasmid#213413PurposeVinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).DepositorInsertVcnCS-E1021A (VCL Chicken, Synthetic)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1021 to alanine (E…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C16orf59-mCer
Plasmid#211424Purposemammalian expression of human C16orf59 C-terminally fused to mCeruleanDepositorInsertC16orf59 (C16orf59 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RecQL4-mCer
Plasmid#211402Purposemammalian expression of human RecQL4 C-terminally fused to mCeruleanDepositorInsertRecQL4 (RECQL4 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mcm2-mCer
Plasmid#211397Purposemammalian expression of human Mcm2 C-terminally fused to mCeruleanDepositorInsertMcm2 (MCM2 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cdc45-mVen
Plasmid#211390Purposemammalian expression of human Cdc45 C-terminally fused to mVenusDepositorInsertCdc45 (CDC45 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cdc45-mCer
Plasmid#211389Purposemammalian expression of human Cdc45 C-terminally fused to mCeruleanDepositorInsertCdc45 (CDC45 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RPA32-mVen
Plasmid#211388Purposemammalian expression of human RPA32 C-terminally fused to mVenusDepositorInsertRPA32 (RPA2 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RPA32-mCer
Plasmid#211387Purposemammalian expression of human RPA32 C-terminally fused to mCeruleanDepositorInsertRPA32 (RPA2 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C17orf53-mCer
Plasmid#211420Purposemammalian expression of human C17orf53 C-terminally fused to mCeruleanDepositorInsertC17orf53 (HROB Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C17orf53_1-87-mCer
Plasmid#211441Purposemammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mCeruleanDepositorInsertC17orf53 (HROB Human)
UseTagsmCeruleanExpressionMammalianMutationDeletion of aa 88-646PromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C17orf53_1-87-mVen
Plasmid#211442Purposemammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mVenusDepositorInsertC17orf53 (HROB Human)
UseTagsmVenusExpressionMammalianMutationDeletion of aa 88-646PromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mcm5-mVen
Plasmid#211394Purposemammalian expression of human Mcm5 C-terminally fused to mVenusDepositorInsertMcm5 (MCM5 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C17orf53-mVen
Plasmid#211421Purposemammalian expression of human C17orf53 C-terminally fused to mVenusDepositorInsertC17orf53 (HROB Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C16orf59-mVen
Plasmid#211425Purposemammalian expression of human C16orf59 C-terminally fused to mVenusDepositorInsertC16orf59 (C16orf59 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApaLIE225A, K227A-HA
Plasmid#211460PurposeExpress mitochondrial localized ApaLI with E225A and K227A mutationsDepositorInsertApaLI-E225A-K227A
UseTagsHAExpressionMammalianMutationE225A, K227APromoterCMVAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5-FRT-TO-Ubc13
Plasmid#212806PurposeConstitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cellsDepositorInsertUbc13
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Mms2
Plasmid#212807PurposeConstitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cellsDepositorInsertMms2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA K230A
Plasmid#210811PurposeTo block the formation of the initial Schiff base with FBPDepositorInsertALDOA (ALDOA Human)
UseTagsHAExpressionMammalianMutationresidues 230 of ALDOA mutated to alaninePromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-PuRRRE
Plasmid#208327PurposeC-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-PuRRRE
UseTagsPuRRREExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-TREER
Plasmid#208325PurposeC-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-TREER
UseTagsTREERExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-TinyERRE
Plasmid#208324PurposeC-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-TinyERRE
UseTagsTinyERREExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-N
Plasmid#210270PurposeN-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.DepositorTypeEmpty backboneUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 BBS pcDNA3
Plasmid#206106Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit with a single Bungarotoxin Binding Site motif inserted after aa549HADepositorInsertcacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only