We narrowed to 2,365 results for: DAP

1. RCAS-sgATG7

   Plasmid

   #75228

   Purpose

   An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

   Depositor

   Noor Gammoh

   Article

   Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

   Insert

   Autophagy Related 7 (Atg7 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pLenti-HF1-PGK-Puro

   Plasmid

   #110857

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-VQRRA-PGK-Puro

   Plasmid

   #110858

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-VRERRA-PGK-Puro

   Plasmid

   #110859

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-VQR-P2A-Puro

   Plasmid

   #110848

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQR
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-VRERRA-P2A-Puro

   Plasmid

   #110852

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYI2216

   Plasmid

   #228349

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0081 expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
   

   Use

   Affinity Reagent/ Antibody

   Tags

   AAA linker followed by FLAG-His6 and MF_ secretio…

   Expression

   Yeast

   Mutation

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNK7185

   Plasmid

   #219765

   Purpose

   Vector for expression PzPKS2 in yeast (P.pastoris)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   PzPKS2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGAP

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNK7181

   Plasmid

   #219766

   Purpose

   Vector for expression HmS in yeast (P.pastoris)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   HmS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGAP

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNK7182

    Plasmid

    #219767

    Purpose

    Vector for expression PpASCL in yeast (P.pastoris)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    PpASCL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNK7188

    Plasmid

    #219768

    Purpose

    Vector for expression Pv4CL1 in yeast (P.pastoris)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    Pv4CL1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-VRER-P2A-Puro

    Plasmid

    #110849

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRER
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-HF1-P2A-Puro

    Plasmid

    #110850

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-VQR-PGK-Puro

    Plasmid

    #110855

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQR
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-VRER-PGK-Puro

    Plasmid

    #110856

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRER
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHH0103_FYB-1/2

    Plasmid

    #91459

    Purpose

    Protein expression and purification of human SH3 domain construct FYB-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FYB-1/2 (FYB1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103_FYB-2/2

    Plasmid

    #91300

    Purpose

    Protein expression and purification of human SH3 domain construct FYB-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FYB-2/2 (FYB1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GST-beta2(616-951)Double

    Plasmid

    #100746

    Purpose

    Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Long isoform with deletion of clathrin binding mo…

    Promoter

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GST-beta2(616-951)Y815A

    Plasmid

    #100744

    Purpose

    Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Long isoform with Y815A mutation

    Promoter

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-FNLS-P2A-GFP-PGK-Puro

    Plasmid

    #110869

    Purpose

    Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge