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pET22-HT/plsA

Plasmid

#73625

Purpose

Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pICH-nuc-roGFP2-ORP1

Plasmid

#191758

Purpose

Ti plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

nuc-roGFP2-ORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-NOP56-C3

Plasmid

#134536

Purpose

Expresses YFP-NOP56 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

NOP56 (NOP56 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV SOCS-3

Plasmid

#11486

Depositor

Ronald Kahn

Article

Ueki et al Mol Cell Biol. 2004 Jun . 24(12):5434-46

Insert

SOCS-3 (Socs3 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4A-FLAG

Plasmid

#15821

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin4A (CUL4A Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N440

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pOZITX-S1

Plasmid

#184925

Purpose

Expresses the catalytic S1 subunit of OZITX (EcPltAB)

Depositor

Jonathan Javitch

Article

Keen et al Commun Biol. 2022 Mar 23;5(1):256. doi:

Insert

codon optimized S1 subunit of OZITX

Expression

Mammalian

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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ArcLight-A242

Plasmid

#36857

Depositor

Vincent Pieribone

Article

Jin et al Neuron. 2012 Sep 6;75(5):779-85.

Insert

fluorescent protein voltage sensor

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

semian CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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ND6-L1397-N_UGI-NES_right

Plasmid

#187846

Purpose

expresses the right-side half ND6-UGI-NES in mammalian cells

Depositor

Chengqi Yi

Article

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–NES–ATP5B 3'UTR

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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ND6-L1397-N_UGI-NES_left

Plasmid

#187845

Purpose

expresses the left-side half ND6-UGI-NES in mammalian cells

Depositor

Chengqi Yi

Article

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–hivNES–SOD2 3'UTR

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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T7RNAP Cyslight

Plasmid

#205077

Purpose

T7RNAP protein expression

Depositor

Smita Patel

Article

Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

Insert

T7RNAP Cyslight (T7p07 Bacteriophage T7)

Expression

Bacterial

Mutation

C125S, C347S, C510S, C515S, C530S, C723S

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry- eMagBF - 5ptaseOCRL

Plasmid

#162254

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

mCherry- eMagBF - 5ptaseOCRL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBWB419

Plasmid

#140637

Purpose

RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

Cas9

Expression

Bacterial

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-deltaBamHI-human MIZ1

Plasmid

#74167

Purpose

Mammalian expression of human MIZ1

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MIZ1 (ZBTB17 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-DN-hCUL5-FLAG

Plasmid

#15823

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin5 (CUL5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N441

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4B-FLAG

Plasmid

#15822

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin4B (CUL4B Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N594

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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Human LFA-1 alpha

Plasmid

#8630

Depositor

Timothy Springer

Article

Larson et al J Cell Biol 1989 Feb;108(2):703-12.

Insert

LFA1 alpha (ITGAL Human)

Expression

Bacterial

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
MSCV-Mycn-IRES-GFP

Plasmid

#35394

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Mycn (Mycn Mouse)
GFP

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 12, 2013

Availability

Academic Institutions and Nonprofits only
Lys-eMagB-iRFP

Plasmid

#162249

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

p18-eMagB-iRFP

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ras-LOCKR-S R89L (negative control)

Plasmid

#216497

Purpose

FRET-based reporter for Ras activity, untargeted, negative control

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Ras-LOCKR-S R89L negative control

Expression

Mammalian

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-C1-CAMKIIbeta

Plasmid

#21227

Depositor

Tobias Meyer

Article

Shen et al Neuron. 1998 Sep . 21(3):593-606.

Insert

CAMKIIbeta (Camk2b Rat)

Tags

GFP

Expression

Mammalian

Mutation

Lab plasmid KS 0002.

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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