We narrowed to 4,853 results for: codon optimized

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pscALPS-SIV-RCM vpx

Plasmid

#115812

Purpose

Encodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance protein

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVRCM Vpx (AF349680)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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SpCas9-ABE8e(V106W)

Plasmid

#198547

Purpose

Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-ABE8e(V106W)

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P.1

Plasmid

#177959

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )

Expression

Mammalian

Mutation

P80R,R203K,G204R

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSL8215 (dCas12f–σE (sigmaE) system, entry vector)

Plasmid

#254272

Purpose

Encodes E. coli codon-optimized dCas12f and σE (sigmaE) from Flagellimonas taeanensis driven by constitutive promoters, with BsaI-containing restriction sites for guide RNA cloning

Depositor

Samuel H. Sternberg

Publication

Hoffmann et al Nature. 2026 Mar 4. doi: 10.1038/s41586-

Inserts

dCas12f
sigma E

Use

CRISPR

Expression

Bacterial

Promoter

J23105

Available since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pJR2955

Plasmid

#122550

Purpose

Expression of a C. elegan codon optimized florescent protein (CemNeptune2.5) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Publication

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemNeptune2.5

Expression

Bacterial

Promoter

Pnhx-2

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJR2956

Plasmid

#122533

Purpose

Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Publication

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemOrange2

Expression

Bacterial

Promoter

Pnhx-2

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pIA1363

Plasmid

#166861

Purpose

Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

Depositor

Irina Artsimovitch

Publication

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp8 (ORF1ab Synthetic)

Tags

His-GB1

Expression

Bacterial

Promoter

T7

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC39A8

Plasmid

#132177

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

Insert

SLC39A8 (SLC39A8 Human)

Expression

Mammalian

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pRW006-SM-destination-pro35S-Cas9

Plasmid

#104439

Purpose

Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

Depositor

Detlef Weigel

Publication

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Plant

Promoter

35S

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQR-P2A-Puro

Plasmid

#110848

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-P2A-Puro

Plasmid

#110852

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-PGK-Puro

Plasmid

#110857

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-PGK-Puro

Plasmid

#110859

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQR-P2A-GFP-PGK-Puro

Plasmid

#110861

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-P2A-GFP-PGK-Puro

Plasmid

#110863

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-P2A-GFP-PGK-Puro

Plasmid

#110865

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-BE3-2X

Plasmid

#110835

Purpose

CMV expression vector for BE3-2X construct (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3-2X

Expression

Mammalian

Mutation

2 tandem NLS sequences in the XTEN linker

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-BE3-FNLS

Plasmid

#110836

Purpose

CMV expression vector for BE3-FNLS construct (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3-FNLS

Tags

3X FLAG

Expression

Mammalian

Mutation

NLS sequence at the N-terminus

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3-P2A-Puro

Plasmid

#110838

Purpose

Lentiviral vector for constitutive expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3-PGK-Puro

Plasmid

#110842

Purpose

Lentiviral vector for constitutive expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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