We narrowed to 5,111 results for: codon optimized

1. pCAG-T3-hCasD10A-pA

   Plasmid

   #51638

   Purpose

   Expresses Cas9-nickase in mammalian cells and zygotes.

   Depositor

   Wataru Fujii

   Article

   Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

   Insert

   Codon-optimized Cas9 D10A
   

   Use

   CRISPR

   Tags

   FLAG, NLS, and polyA

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pALPS tat-P2A-rev

   Plasmid

   #101331

   Purpose

   Lentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3 tat and rev
   

   Use

   Lentiviral

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJFRC7-UAS-NPRR_ANP

   Plasmid

   #133243

   Purpose

   Fluorescent reporter for neuropeptide release imaging

   Depositor

   David Anderson

   Article

   Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

   Insert

   rat atrial natriuretic peptide-fused codon-optimized GCaMP6s
   

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGB-SpCas9

   Plasmid

   #210533

   Purpose

   Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   SpCas9
   

   Use

   Synthetic Biology

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ155

   Plasmid

   #69306

   Purpose

   Gateway entry vector with AteCas9D10A

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   AteCas9D10A (Arabidopsis codon optimized)
   

   Use

   CRISPR

   Mutation

   D10A mutation; NLS fusion

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCas13 Twin-SUMO

   Plasmid

   #199753

   Purpose

   Expresses codon optimized miniature mCas13 for protein expression in E.coli

   Depositor

   Magdy Mahfouz

   Article

   Mahas et al ACS Synth Biol. 2021 Oct 15;10(10):2541-

   Insert

   mCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Promoter

   LacI

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSZT025

   Plasmid

   #140033

   Purpose

   KO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

   Depositor

   Peter Nixon

   Article

   Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

   Insert

   Truncated thioesterase gene
   

   Expression

   Bacterial

   Promoter

   cLac143

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS403red-­luxTA2-­4F

   Plasmid

   #166030

   Purpose

   Expresses luxTA(2-­4F) mutant

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   luxR
   

   Use

   Synthetic Biology

   Tags

   Nuclear localization signal and three copies of V…

   Expression

   Yeast

   Mutation

   S116Y, W201R, codon-optimized for expression in y…

   Promoter

   pPGK1 mutant

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS405red-­phlTA2-­1E

   Plasmid

   #166029

   Purpose

   Expresses phlTA(2-­1E) mutant

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   phlF
   

   Use

   Synthetic Biology

   Tags

   Nuclear localization signal and three copies of V…

   Expression

   Yeast

   Mutation

   P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

   Promoter

   pPGK1 mutant

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGK415-­luxTA2-­4F

    Plasmid

    #165971

    Purpose

    Expresses luxTA(2-­4F) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    luxR
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    S116Y, W201R, codon-optimized for expression in y…

    Promoter

    pPGK1 mutant

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Blat-BSD

    Plasmid

    #163791

    Purpose

    The plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.

    Depositor

    Yongming Wang

    Article

    Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p33Fn

    Plasmid

    #158155

    Purpose

    Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Rice codon optimized

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p1148-pCSDest-AcrIIA4-mCherry

    Plasmid

    #126204

    Purpose

    Mammalian expression of AcrIIA4 fused to mCherry

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    codon-optimized AcrIIA4
    

    Tags

    FLAG/NLS and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSN074

    Plasmid

    #166731

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pALPS_3'LTR GFP@gag start SFFV rev

    Plasmid

    #101339

    Purpose

    LTR drives GFP and internal SFFV promoter drives codon optimized rev

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP, rev
    

    Use

    Lentiviral

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGK415-­phlTA2-­1E

    Plasmid

    #165970

    Purpose

    Expresses phlTA(2-­1E) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    phlF
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

    Promoter

    pPGK1 mutant

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-SERTG56A -myc

    Plasmid

    #107459

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G56A conferring wildtype phenotype

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Expression

    Mammalian

    Mutation

    G56A

    Promoter

    CMV

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pALPS_3'LTR GFP@gag start SFFV tat

    Plasmid

    #101338

    Purpose

    LTR drives GFP and internal SFFV promoter drives codon optimized tat

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP, tat
    

    Use

    Lentiviral

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ159

    Plasmid

    #69308

    Purpose

    Gateway entry vector with hSpCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    hSpCas9D10A (Human codon optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Mutation

    D10A mutation; NLS fusion

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSN069

    Plasmid

    #166728

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-Mxi1
    

    Use

    CRISPR

    Tags

    Mxi1

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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