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pDiGi

Plasmid

#59323

Purpose

Inducible expression of dsRed and GFP in bacteria

Depositor

Sophie Helaine, David Holden

Article

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Expression

Bacterial

Promoter

Pbad and Plac

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pQE30-His-IF3

Plasmid

#124125

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Initiation factor 3 (infC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-His-RF3

Plasmid

#124131

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 3 (prfC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-TrpRS-His

Plasmid

#124120

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tryptophanyl-tRNA ligase (trpS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-GlnRS-His

Plasmid

#124108

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutaminyl-tRNA ligase (glnS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-His-IleRS

Plasmid

#124112

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Isoleucyl-tRNA ligase (ileS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAB1

Plasmid

#62547

Purpose

broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

Depositor

Michael Franklin

Article

Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

Insert

eGFP

Use

Broad host range plasmid

Expression

Bacterial

Promoter

pTrc

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Foxp1A

Plasmid

#16362

Depositor

Anjana Rao

Article

Hu et al Nat Immunol. 2006 Aug . 7(8):819-26.

Insert

FOXP1A (Foxp1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pg-HIF-1alpha-EGFP

Plasmid

#87204

Purpose

C-terminally EGFP-tagged human HIF-1a

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

HIF-1a (HIF1A Human)

Tags

EGFP

Expression

Mammalian

Mutation

L40F, M88V, Q814R

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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1.5 BV

Plasmid

#16048

Depositor

Robert Benezra

Article

Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

Insert

Id1 promoter region (Id1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

-1585 to +88 of promoter

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA4TO-24xGCN4_v4-BFP-24xPP7

Plasmid

#74929

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-BFP-24xPP7

Expression

Mammalian

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCW57.1_AOX-FLAG

Plasmid

#177984

Purpose

All-in-one dox-inducible expression of Alternative Oxidase

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

AOX

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Codon optimized cDNA for expression in human cells

Promoter

TRE

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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GLUT1-GFP

Plasmid

#89440

Purpose

GLUT1 construct

Depositor

Jeffrey Rathmell

Article

Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

Insert

GLUT1 (Slc2a1 Rat)

Tags

GFP

Expression

Mammalian

Promoter

EF-1a

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF1a-GATA1-IRES-Puro

Plasmid

#61062

Purpose

lentiviral vector for GATA1

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

GATA1 (GATA1 Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1alpha

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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1119 pBabe puroL HA FKHR AAA

Plasmid

#9025

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR AAA (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

T24A S256A S319A

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pQP-T2A

Plasmid

#102583

Purpose

Light-controlled activation of expression

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

Insert

BphP1-VP16-T2A-NLS-GAL4-QPAS1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pL

Plasmid

#196286

Purpose

35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV L antigenome encoding codon-optimized L (RdRp)

Use

CRISPR

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLL3.7 EGFPC2 TAZ

Plasmid

#66850

Purpose

Express GFP-fused TAZ by lentivirus

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

WWTR1/TAZ (WWTR1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a WT DBM (H212R)

Plasmid

#8352

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

H212R

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pDXinit

Plasmid

#110101

Purpose

E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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