We narrowed to 4,333 results for: 1-Oct;

1. pBW2115_pCAG-PV1-PhiC31-N233-L1-GID1-NLS-BGHpA

   Plasmid

   #108805

   Purpose

   Expresses N-terminus to aa233 of PhiC31 integrase fused to GID1 CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment N-terminus to aa233
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBW2116_pCAG-PV1-GAI-L1-PhiC31-234C-NLS-BGHpA

   Plasmid

   #108806

   Purpose

   Expresses aa234 to C-terminus of PhiC31 integrase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa234 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. USPL1cat-pEYFP_C1

   Plasmid

   #85766

   Purpose

   mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, catalytic domain (aa 212-514) (USPL1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   catalytic domain (aa 212-514)

   Promoter

   CMV

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBW1349_pCAG-PV1-PhiC31-N571-L1-FRB-NLS-BGHpA

   Plasmid

   #108795

   Purpose

   Expresses N-terminus to aa571 of PhiC31 integrase fused to FRB CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment N-terminus to aa571
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBW1362_pCAG-PV1-FKBP-L1-PhiC31-572C-NLS-BGHpA

   Plasmid

   #108796

   Purpose

   Expresses aa572 to C-terminus of PhiC31 integrase fused to FKBP CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa572 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FKBP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLL5.0 mCherry-hCortKD-Flcortmut3YD

   Plasmid

   #187266

   Purpose

   Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

   Depositor

   Alpha Yap

   Article

   Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

   Insert

   Cortactin (CTTN Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   3YD (Y412, Y470, Y486)

   Promoter

   U6, 5'LTR

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLL5.0 hCortKD-Flcortmut3YF mCherry

   Plasmid

   #187265

   Purpose

   Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

   Depositor

   Alpha Yap

   Article

   Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

   Insert

   Cortactin (CTTN Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   3YF (Y412, Y470, Y486)

   Promoter

   U6, 5'LTR

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ATP1A1_G6_T804N_v1_Dual_epegRNA_tevopreQ1

   Plasmid

   #187458

   Purpose

   Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 epegRNA (tevopreQ1) with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G6 T804N epegRNA (tevopreQ1) (ATP1A1 Human)
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTox-EGFPsite1_NGG-PAM (BPK740)

   Plasmid

   #181746

   Purpose

   p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)
   

   Use

   Selection plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

    Plasmid

    #108769

    Purpose

    Expresses N-terminus to aa396 of Flp recombinase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment N-terminus to aa396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRevTRE MKL1 S449A/T450A/S454A

    Plasmid

    #19847

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S449A/T450A/S454A (MRTFA Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    changed Serine 449, Threonine 450 and Serine 454 …

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pProEx-HTa-SOD1 C57D/C146D

    Plasmid

    #191851

    Purpose

    express C57D/C146D mutant SOD1 in bacterial cells

    Depositor

    Nam-Chul Ha

    Article

    Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C57D, C146D

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCWXPGR-pTF-DVL2deltaDIX

    Plasmid

    #114276

    Purpose

    TET-inducible expression of DVL2 protein with a deletion of its DIX-domain

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    DVL2 (Dvl2 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    deletion of DIX domain

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid

    #92102

    Purpose

    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpA

    Plasmid

    #108814

    Purpose

    Expresses aa327 to C-terminus of TP901 integrase fused to FKBP

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    TP901 integrase fragment aa327 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

    Plasmid

    #69219

    Purpose

    Expresses wild type SpCas9 fused to Zif268 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    Zif268 (Egr1 Mouse)
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCin4-3xFLAG-MKL1 S449A/T450A/S454A

    Plasmid

    #19845

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S449A/T450A/S454A (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 449, Threonine 450 and Serine 454 …

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pLL5.0 hCortKD-Flcortmut3YF EGFP

    Plasmid

    #187263

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    3YF (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBW2660_pCAG-PV1-iCre-N270-L1-FRB-NLS-BGHpA

    Plasmid

    #108737

    Purpose

    Expresses N-terminus to aa270 of Cre recombinase fused to FRB CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment N-terminus to aa270
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPRIME-CMV-GFP-shDyn2

    Plasmid

    #132712

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shDyn(2) (Pdyn Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge