We narrowed to 2,462 results for: LARGE

1. p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225)

   Plasmid

   #224534

   Purpose

   A Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tag

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-mNeonGreen-HA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-IRES-mTagBFP2-pA (JDW 1361)

   Plasmid

   #224535

   Purpose

   A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-FLAG-NLS-mTagBFP2-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221-Lifeact- mScarlet-HA (JDW 1247)

   Plasmid

   #224538

   Purpose

   A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Lifeact-mScarlet-HA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME H2B mCerulean (JDW 1150)

   Plasmid

   #224543

   Purpose

   A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   H2B-mCerulean
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5E 5X C120 c-fos min pro (JDW 1241)

   Plasmid

   #224544

   Purpose

   A Gateway compatible 5' entry clone containing 5 TAEL transcription factor binding sites and a minimal c-Fos promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   5x-C120-c-Fos promoter
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pS238D1::sptse5-CT

   Plasmid

   #192959

   Purpose

   For expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studies

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1317)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326)

   Plasmid

   #229812

   Purpose

   A CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   nls-mCherry
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcsII-LMP7-flag

   Plasmid

   #86773

   Purpose

   C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   immunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME Lamin B Vhh mNeonGreen-pA (JDW 1312)

   Plasmid

   #224501

   Purpose

   Gateway compatible middle entry clone containing Lamin B nanobody fused to mNeonGreen with HA Tag (For visualizing the nuclear lamina, lamin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Lamin B Vhh mNeonGreen-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. WDV-Echistatin

    Plasmid

    #226985

    Purpose

    Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

    Depositor

    Wendy Gordon

    Article

    Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

    Inserts

    WDV (repA )
    Echistatin
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

    Plasmid

    #224489

    Purpose

    Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Sun1-2xsfGFP-6xMYC-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

    Plasmid

    #224500

    Purpose

    Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-mNeonGreen-HA-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p5E-MCS-c-Fos-b-globin (JDW 1237)

    Plasmid

    #229829

    Purpose

    A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    c-fos minimal promoter and b-globin intron
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pME Actin Vhh Halo Tag (JDW 1223)

    Plasmid

    #224498

    Purpose

    Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-Halo-Tag
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcsII-pb4-flag

    Plasmid

    #86768

    Purpose

    C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    proteasome beta subunit 4 (PSMB4 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pME-3xNLS-(G2SG2)2-Cre (JDW 1149)

    Plasmid

    #224514

    Purpose

    A Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to Cre

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xNLS-(G2SG2)2-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKTop::tse5-CT (1169-1281)

    Plasmid

    #192955

    Purpose

    For membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    tse5-CT (1169-1281)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1281)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)

    Plasmid

    #229827

    Purpose

    A CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xnls-TagBFP2-MCS-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p5E-Dll4-F2-E1B-B-Globin (JDW 1366)

    Plasmid

    #229843

    Purpose

    A gateway compatible 5' entry clone containing the murine Dll4 F2 arterial specific enhancer upstream of a minimal E1b promoter and intron for arterial and endocardial expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Dll4-F2 (exon3)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)

    Plasmid

    #229811

    Purpose

    A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA transactivator-LexA-Operon-nls-GFP
    

    Use

    Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge