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pMRX-IZ-ANXA5-mRuby3

Plasmid

#184891

Purpose

Stable expression of ANXA5-mRuby3 (Annexin A5-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA5 (ANXA5 Human)

Tags

mRuby3

Expression

Mammalian

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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JDW 1043 (pLenti6.2_CMV_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#233538

Purpose

A CMV driven human LEF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Lentiviral

Tags

V5

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1050 (pME_V5_GATA1_P2A_H2A_mCherry)

Plasmid

#232127

Purpose

A middle entry gateway clone containing V5 tagged human GATA1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA1 (GATA1 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA4-mRuby3

Plasmid

#184890

Purpose

Stable expression of ANXA4-mRuby3 (Annexin A4-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA4 (ANXA4 Human)

Tags

mRuby3

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDendra2-Hygro

Plasmid

#202407

Purpose

Dendra2 expression plasmid

Depositor

Morito Kurata

Article

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

Hygromycin

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3HA-Rab3il1

Plasmid

#246267

Purpose

Rab8 GEF

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

3HA-Rab3il1 (Rab3il1 Rat)

Tags

3HA

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3HA-TBC1D15

Plasmid

#246262

Purpose

Rab7 GAP

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

3HA-TBC1D15 (Tbc1d15 Mouse)

Tags

3HA

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pmRNA-iEMCV-EGFP-A120

Plasmid

#225905

Purpose

In vitro transcription of cap-independently (EMCV IRES) translated mRNA encoding EGFP.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

A-inserted T7 class III Φ6.5 promoter
EMCV IRES
EGFP
2x β-globin UTR
poly(A) tail

Use

Synthetic Biology; Mrna preparation

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pmRNA-iHCV-EGFP-A120

Plasmid

#225906

Purpose

In vitro transcription of cap-independently (HCV IRES) translated mRNA encoding EGFP.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

A-inserted T7 class III Φ6.5 promoter
HCV IRES
EGFP
2x β-globin UTR
poly(A) tail

Use

Synthetic Biology; Mrna preparation

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-Rab5a(S34N)

Plasmid

#246255

Purpose

Rab5a (Dominant negative)

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mEGFP-Rab5a(S34N) (Rab5a Rat)

Expression

Mammalian

Mutation

S34N

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-Rab5a(Q79L)

Plasmid

#246254

Purpose

Rab5a (Constitutively active)

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mEGFP-Rab5a(Q79L) (Rab5a Rat)

Expression

Mammalian

Mutation

Q79L

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mEGFP-Rab7a

Plasmid

#246263

Purpose

Rab7 sensor donor

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mEGFP-Rab7a (Rab7a Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mEGFP-Rab10

Plasmid

#246281

Purpose

Rab10 sensor donor

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mEGFP-Rab10 (Rab10 Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mEGFP-Rab10(Q68L)

Plasmid

#246273

Purpose

Rab10 (Constitutively active)

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mEGFP-Rab10(Q68L) (Rab10 Rat)

Expression

Mammalian

Mutation

Q68L

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTurquoise2-Rab10(Q68L)

Plasmid

#246272

Purpose

Rab10 (Constitutively active)

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mTurquoise2-Rab10(Q68L) (Rab10 Rat)

Expression

Mammalian

Mutation

Q68L

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTurquoise2-Rab10(T23N)

Plasmid

#246274

Purpose

Rab10 (Dominant negative)

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mTurquoise2-Rab10(T23N) (Rab10 Rat)

Expression

Mammalian

Mutation

T23N

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mEGFP-Rab4a(S27N)

Plasmid

#246253

Purpose

Rab4a (Dominant negative)

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mEGFP-Rab4a(S27N) (Prph2 Rat)

Expression

Mammalian

Mutation

S27N

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mEGFP-Rab7a(Q67L)

Plasmid

#246265

Purpose

Rab7a (Constitutively active)

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mEGFP-Rab7a(Q67L) (Rab7a Rat)

Expression

Mammalian

Mutation

Q67L

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mEGFP-Rab4a(Q72L)

Plasmid

#246252

Purpose

Rab4a (Constitutively active)

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mEGFP-Rab4a(Q72L) (Rabggtb Rat)

Expression

Mammalian

Mutation

Q72L

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3HA-Rab3ip

Plasmid

#246268

Purpose

Rab8 GAP

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

3HA-Rab3ip (Rab3ip Rat)

Tags

3HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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