We narrowed to 1,249 results for: vent

1. pHA853 - mec-4p::Fynempty

   Plasmid

   #139210

   Purpose

   Control vector for Fyn kinase expression in mec-4 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   deletion and frame shift to prevent Fyn expression

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHA851 - osm-10p::Fynempty

   Plasmid

   #139208

   Purpose

   Control vector for Fyn kinase expression in osm-10 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   deletion and frame shift to prevent Fyn expression

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. KG#84

   Plasmid

   #110878

   Purpose

   Expresses the C. elegans acy--1 P260S gain-of-function cDNA in ventral cord cholinergic motor neurons

   Depositor

   Kenneth Miller

   Article

   Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

   Inserts

   unc-17beta promoter
   acy-1 P260S gain-of-function cDNA
   unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Changed Proline to Serine at amino acid 260

   Promoter

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9-KRAB

   Plasmid

   #110820

   Purpose

   Conventional dCas9 repressor-dCas9-KRAB

   Depositor

   George Church, Alejandro Chavez

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

   Plasmid

   #154786

   Purpose

   Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE

   Depositor

   Robert Singer

   Article

   Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

   Insert

   Adenosine deaminse (Adar Synthetic, Fly)
   

   Use

   Lentiviral

   Tags

   GFP, V5, and mCherry

   Expression

   Mammalian

   Mutation

   Synonymized serine to prevent autoediting of ADAR…

   Promoter

   UBC

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

   Plasmid

   #154787

   Purpose

   Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain

   Depositor

   Robert Singer

   Article

   Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

   Insert

   Adenosine deaminse (Adar Synthetic, Fly)
   

   Use

   Lentiviral

   Tags

   GFP, V5, and stdMCP

   Expression

   Mammalian

   Mutation

   Synonymized serine to prevent autoediting of ADAR…

   Promoter

   UBC

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpySwitch

   Plasmid

   #184225

   Purpose

   Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   SpySwitch
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E77A mutation prevents isopeptide bond formation,…

   Promoter

   T7

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIZ-EGFP-MYO10-HMM

   Plasmid

   #87257

   Purpose

   Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO10 (Myo10 Mouse)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Myo10 is truncated to contain aa.1-941

   Promoter

   OpIE2

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRLNeo-pEF1a-p53ash-mCerulean

   Plasmid

   #69579

   Purpose

   Expresses p53 tagged with mCerulean

   Depositor

   Galit Lahav

   Article

   Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

   Insert

   p53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   7 silent mutations in p53 DNA to prevent targetin…

   Promoter

   EF1alpha

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC

    Plasmid

    #69583

    Purpose

    Expresses p53 tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    7 silent mutations in p53 DNA to prevent targetin…

    Promoter

    EF1alpha

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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