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pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

Plasmid

#67979

Purpose

Cas9 activity reporter (control) with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72666

Purpose

Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72667

Purpose

Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-BsdCas9-W

Plasmid

#67978

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Promoter

EF1a

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

Plasmid

#67985

Purpose

Cas9 activity reporter (control) with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherryABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

Plasmid

#67991

Purpose

CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-YFP-KRAS4B

Plasmid

#112718

Purpose

Expresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.

Depositor

Kenneth Westover

Article

Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

Insert

YFP-KRAS4B (KRAS Human)

Expression

Mammalian

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

Plasmid

#67976

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmAG cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGmCherry-gRNA

Plasmid

#87110

Purpose

gRNA cloning vector with CAGmCherry

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

Plasmid

#67975

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AZsG cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pC0043-PspCas13b-gRNA LINE1

Plasmid

#223699

Purpose

gRNA of dPspCas13b-FTO targeting LINE1

Depositor

Chuan He

Article

Wei et al Science. 2022 May 27;376(6596):968-973.

Insert

gRNA target sequence for LINE1

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

Plasmid

#67984

Purpose

Cas9 activity reporter with GFP and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

Plasmid

#67990

Purpose

CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CFP-KRAS4B

Plasmid

#112717

Purpose

Expresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.

Depositor

Kenneth Westover

Article

Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

Insert

CFP-KRAS4B (KRAS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

Plasmid

#67983

Purpose

Cas9 activity reporter (control) with GFP and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Pet22b-mEGFP-YAP-N-IDR

Plasmid

#166440

Purpose

Bacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP-N-IDR(1-164) (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRV-RB

Plasmid

#106374

Purpose

creating stable line with RFP and BSD resistance

Depositor

Tong-Chuan He

Article

Liao et al Gene Ther. 2017 Jun;24(6):333-341. doi:

Insert

mRFP and blasticidin resistance gene

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRV-GN

Plasmid

#106373

Purpose

creating stable line with GFP and G418 resistance

Depositor

Tong-Chuan He

Article

Liao et al Gene Ther. 2017 Jun;24(6):333-341. doi:

Insert

eGFP and geneticin/G418 resistance gene

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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Pet22b-mCherry-TEAD4-IDR

Plasmid

#166442

Purpose

Bacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, TEAD4-IDR (TEAD4 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Pet22b-mEGFP-YAP-C-IDR

Plasmid

#166441

Purpose

Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP-C-IDR(266-504) (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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