We narrowed to 29,789 results for: LIS

1. pSynapsin_NLS_GFP_P2A_His6_GluA2

   Plasmid

   #170453

   Purpose

   Expresses histidine tagged GluA2 in neuronal cells

   Depositor

   Shigeki Watanabe

   Article

   Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

   Insert

   GluA2 (Gria2 Mouse)
   

   Use

   Lentiviral

   Tags

   Hexa-histidine

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. dCas9-ddMSK1

   Plasmid

   #165603

   Purpose

   Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)

   Depositor

   Isaac Hilton

   Article

   Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

   Insert

   S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 S. Pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. FAB(PX-LD)

   Plasmid

   #214426

   Purpose

   Detection of peroxisome-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   FKBP-V5
   

   Use

   Tags

   CFAST-Halo and FKBP-V5-RspAN

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNICKclos2.0

   Plasmid

   #73228

   Purpose

   Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

   Inserts

   Cas9 nickase
   sgRNA to xylR
   

   Use

   E.coli - clostridium shuttle vector

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Pj23119 and Pthl

   Available since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSynapsin_NLS_GFP-P2A_SNAP_GluA2

   Plasmid

   #170455

   Purpose

   Expresses SNAP tagged GluA2 in neuronal cells

   Depositor

   Shigeki Watanabe

   Article

   Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

   Insert

   GluA2 (Gria2 Mouse)
   

   Use

   Lentiviral

   Tags

   SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Rescue Septin 11 mRNA

   Plasmid

   #122022

   Purpose

   Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

   Depositor

   Angel de Blas

   Article

   Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

   Insert

   SEPT11 (Sept11 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Silent mutations are in 5 consecutive codons (AUU…

   Promoter

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATG9A-EGFP

   Plasmid

   #210367

   Purpose

   Expresses ATG9A fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   ATG9A (ATG9A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1518_pAAV-U6-SA-eGFP-gRNA-HLP-SACas9-HA-OLLAS-spA

   Plasmid

   #109316

   Purpose

   Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against eGFP

   Depositor

   William Lagor

   Article

   Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

   Insert

   eGFP gRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSH-EFIRES-P-Seipin-miniIAA7-3XFlag

   Plasmid

   #129722

   Purpose

   AAVS1 safe harbor targeting vector expressing miniIAA7-3XFlag tagged Seipin

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   Seipin (BSCL2 Human)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   miniIAA7-3XFlag

   Expression

   Mammalian

   Mutation

   silent mutations

   Promoter

   EF1a

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-DIO {hCAR}off-{ChETA}on-WPRE

    Plasmid

    #111388

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    ChETA
    

    Use

    AAV

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE

    Plasmid

    #111395

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    DTR-EGFP
    

    Use

    AAV

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. mMib1-FLAG

    Plasmid

    #37116

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    Mib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1)) (Mib1 Mouse)
    

    Use

    Tags

    3 x FLAG

    Expression

    Mammalian

    Mutation

    ACC Kozak sequence added before ATG annotated.

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. NSP6-Flag

    Plasmid

    #210345

    Purpose

    Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-dCpf1-eBE

    Plasmid

    #107688

    Purpose

    Expresses dLbCpf1-eBE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-eBE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCpf1(D832A/E925A/D1148A)

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-His6-SUMO-cSrc-SH2-AviTag

    Plasmid

    #214230

    Purpose

    Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75

    Insert

    cSrc kinase SH2 domain (SRC Human)
    

    Use

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. NFAST(low)-mApple-6xHp

    Plasmid

    #214412

    Purpose

    Lipid droplet localization of low-affinity NFAST fused to mApple-6xHp

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspA-N
    

    Use

    Tags

    mApple-6xHp

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-SH3(5R)

    Plasmid

    #112154

    Purpose

    Mammalian expression plasmid for SH3-5R in a vector expressing mCherry.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3-5R (NCK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    only the second SH3 domain is expressed in this c…

    Promoter

    CMV

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-V0D1

    Plasmid

    #210348

    Purpose

    Expresses ATP6V0D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    V-ATPase V0D1 (ATP6V0D1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSynapsin_NLS_GFP_P2A_SNAP_NR1

    Plasmid

    #170458

    Purpose

    Expresses SNAP tagged NR1 in neuronal cells

    Depositor

    Shigeki Watanabe

    Article

    Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLX317-hcRED

    Plasmid

    #115440

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    hcRED
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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