We narrowed to 11,711 results for: pcDNA

1. pcDNA5/FLAG-HA-DGCR8

   Plasmid

   #106251

   Purpose

   Express DGCR8 in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Chang et al Genome Res. 2015 Sep;25(9):1401-9. doi:

   Insert

   DiGeorge Syndrome Critical Region 8 (DGCR8 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY011 (pcDNA3.1-hLb2Cpf1)

   Plasmid

   #69983

   Purpose

   Expresses humanized Lb2Cpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hLb2Cpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcdna3 Flag Jip1b

   Plasmid

   #52123

   Purpose

   a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

   Depositor

   Roger Davis

   Article

   Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

   Insert

   JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT/TO HIS

   Plasmid

   #19446

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   HIS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HIS tag inserted to create new vector

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4-Beclin1 1-242

   Plasmid

   #24391

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-242.

   Promoter

   Available since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-flag-YTHDF2-N

   Plasmid

   #52302

   Purpose

   Flag-tagged YTHDF2 N-terminal domain, made by mutating to E384(GAA) to stop codon(TAA)

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Changed E384 to stop codon

   Promoter

   T7

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-myc-Ataxin3Q28

   Plasmid

   #22125

   Depositor

   Henry Paulson

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA 3.3 HA-hTSPAN12

   Plasmid

   #115784

   Purpose

   Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   TSPAN12 (TSPAN12 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   multiple silent mutations introduce restriction s…

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNAintron-LASV-GPC

   Plasmid

   #215690

   Purpose

   Produces the Lassa virus glycoprotein

   Depositor

   Melinda Brindley

   Article

   Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

   Insert

   LASV GP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA5-FRT-TO-Nsp10-HF

    Plasmid

    #157708

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp10-HF (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3.1_C-6His-Stx17

    Plasmid

    #112259

    Purpose

    C-term His tagged pcDNA3.1 vector expressing mouse Stx17 (syntaxin 17)

    Depositor

    Madhulika Tripathi

    Insert

    syntaxin 17 (Stx17 Mouse)
    

    Use

    Tags

    6His Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

    Plasmid

    #169332

    Purpose

    Nuclear expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag9 (H2BC21 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-TO-PV-NES-DsRed

    Plasmid

    #16339

    Depositor

    Anton Bennett

    Article

    Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

    Insert

    PV-NES-DsRed (Pvalb Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-ccdB-Renilla

    Plasmid

    #87065

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal Renilla luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Mito-CaNAR2

    Plasmid

    #64731

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal 30 amino acids from DAKAP1,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3/CyclinB1-GFP (28)

    Plasmid

    #39871

    Depositor

    Jonathon Pines

    Article

    Jackman et al Mol Biol Cell. 2002 Mar;13(3):1030-45.

    Insert

    CyclinB1 (CCNB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    V5A

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-myc3-Tsc1

    Plasmid

    #19955

    Depositor

    Yue Xiong

    Article

    Shumway et al J Biol Chem. 2003 Jan 24. 278(4):2089-92

    Insert

    Tsc1 (Tuberous sclerosis 1) (TSC1 Human)
    

    Use

    Tags

    Myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3/Myc-DNMT3B3

    Plasmid

    #37546

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3B3 (DNMT3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-TO-Vpr-mRuby3-IN

    Plasmid

    #167584

    Purpose

    For producing fluorescently labeled HIV-1; expresses HIV-1 integrase fused to Vpr for packaging into HIV-1 and the fluorophore mRuby3

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    Vpr-mRuby3-IN
    

    Use

    Tags

    Vpr-mRuby3

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-β2AR-μDpTT

    Plasmid

    #182326

    Purpose

    Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    beta2AR-μDronpa-TagRFP-T (ADRB2 Human)
    

    Use

    Tags

    Full-length human beta2 adrenergic receptor

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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