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pKJ275

Plasmid

#217491

Purpose

RADARSv3 driving luciferase

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

gaussia luciferase

Expression

Mammalian

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_ALFA-tag-SNAP

Plasmid

#136628

Purpose

Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Myc-PSF-WT

Plasmid

#35183

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pYZ463

Plasmid

#187971

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxGold

Plasmid

#229948

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk1

Plasmid

#81010

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.F102Y.Y137L

Plasmid

#112177

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Expression

Mammalian

Mutation

F102Y.Y137L

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Rtf1

Plasmid

#11060

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Rtf1 (RTF1 Human)

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pYZ038

Plasmid

#200227

Purpose

universal template to build tRNA-gRNA array for multiplex editing CREEPY

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

tRNA-gRNA scaffold

Use

Synthetic Biology

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRK7-FLAG-TSC1

Plasmid

#8995

Depositor

John Blenis

Article

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC1 (TSC1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pRK5R MCS+

Plasmid

#211354

Purpose

empty vector control

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-biRhoBAST

Plasmid

#196344

Purpose

Overexpression of circular biRhoBAST

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

Tornado-biRhoBAST

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGDP1 fosA

Plasmid

#112889

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

fosA

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-tetoff-mVenus-PICdc42

Plasmid

#91876

Purpose

PI(WT): Photo inhibitable (blue light sensitive)

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Tags

mVenus

Expression

Bacterial and Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform II-EGFP

Plasmid

#120316

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the C-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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GST-Pin1

Plasmid

#19027

Depositor

Michael Yaffe

Article

Yaffe et al Science. 1997 Dec 12. 278(5345):1957-60.

Insert

Pin1 (PIN1 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pYZ462

Plasmid

#187970

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pYZ960

Plasmid

#187972

Purpose

TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[T22N]

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[Q67L]

Plasmid

#24900

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[Q67L]

Tags

GFP

Expression

Mammalian

Mutation

Changed Glutamine 67 to Leucine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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