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mApple-Keratin-17
Plasmid#54915PurposeNote- This plasmid contains Keratin 18. Localization: Intermediate Filaments, Excitation: 568, Emission: 592DepositorInsertKeratin (KRT18 Human)
UseTagsmAppleExpressionMammalianMutationV282LPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
mApple-FYN2-N-10
Plasmid#54904PurposeLocalization: Actin/Focal Adhesions, Excitation: 568, Emission: 592DepositorInsertFYN2 (FYN Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
FL-DYSF-pUC57
Plasmid#60177PurposePlasmid contains full-length human dysferlin in a multiple cloning site of pUC57-Kan and can be easily cloned into other vectors.DepositorInsertDysferlin (DYSF Human)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZNF436.1.0-gDNA
Plasmid#113767PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF436DepositorInsertZNF436 (ZNF436 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCitrine-Gamma-Tubulin-17
Plasmid#56560PurposeLocalization: Centrosomes, Excitation: 516, Emission: 529DepositorInsertGamma-Tubulin (TUBG1 Human)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCitrine-LaminB1-10
Plasmid#56562PurposeLocalization: Nuclear Envelope, Excitation: 516, Emission: 529DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
PS-CFP2-Alpha-5-Integrin-12
Plasmid#62794PurposeLocalization: Focal Adhesions, Excitation:400 / 490, Emission:468 / 511DepositorInsertAlpha5Integrin (ITGAV Human)
UseTagsCFPExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagRFP-T2-Actin-7
Plasmid#58032PurposeLocalization: Actin, Excitation: 555, Emission: 584DepositorInsertActin (ACTB Human)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZBTB17.1.0-gDNA
Plasmid#112483PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB17DepositorInsertZBTB17 (ZBTB17 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-ER-4
Plasmid#55922PurposeLocalization: Endoplasmic Reticulum, Excitation: 584, Emission: 607DepositorInsertER (CALR Human)
UseTagsmRFP1ExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
hACTB-RFP-Kir7.1
Plasmid#107179PurposeExpression of N-terminal fused Kir7.1 in mammalian cells.DepositorInsertKCNJ13 (KCNJ13 Human)
UseTagsRFPExpressionMammalianMutationPromoterhACTBAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CNN3A-c002
Plasmid#175456PurposeProtein expression in bacterial cells. Entire CDS, M1-Y329.DepositorInsertCNN3 (CNN3 Human)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIC146
Plasmid#63069PurposeRetroviral expression of EGFP-TEV-S-tag CENP-P for protein localization and affinity purification (LAP)DepositorInsertCENP-P, IMAGE clone 5554572 (CENPP Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
R777-E193 Hs.PRKAA2
Plasmid#70477PurposeGateway ORF clone of human PRKAA2 [NM_006252.3] with stop codon (for native or N-terminal fusions)DepositorInsertPRKAA2 (PRKAA2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pZFX.1.0-gDNA
Plasmid#112462PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZFXDepositorInsertZFX (ZFX Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
mStrawberry-ER-3
Plasmid#55944PurposeLocalization: Endoplasmic Reticulum, Excitation: 574, Emission: 596DepositorInsertER (CALR Human)
UseTagsmStrawberryExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAPSS2
Plasmid#25539PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPAPSS2 (PAPSS2 Human)
UseTagsHisExpressionBacterialMutationContains kinase domain (residues 21-218)PromoterAvailable sinceAug. 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSPDEF.1.0-gDNA
Plasmid#113796PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SPDEFDepositorInsertSPDEF (SPDEF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
R777-E081 Hs.EXOC8
Plasmid#70365PurposeGateway ORF clone of human EXOC8 [NM_175876.4] with stop codon (for native or N-terminal fusions)DepositorInsertEXOC8 (EXOC8 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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NR1D2
Plasmid#25186PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertNR1D2_07 (NR1D2 Human)
UseBaculovirus expressionTagsHisExpressionMutationPromoterpolyhedrinAvailable sinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pResQ shFEN3 3XF-FEN1 D181A
Plasmid#17753DepositorInsertFlap Endonuclease I (FEN1 Human)
UseLentiviral and RNAiTagsTriple FlagExpressionMammalianMutationD181APromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
IDG_CLCA2_OE_1
Plasmid#161645PurposeOverexpresses CLCA2DepositorInsertCLCA2 (CLCA2 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Dronpa-LaminB1-10
Plasmid#57282PurposeLocalization: Nuclear Envelope, Excitation: 503, Emission: 518DepositorInsertLaminB1 (LMNB1 Human)
UseTagsDronpaExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
IDG_CACNA2D4_OE_1
Plasmid#161646PurposeOverexpresses CACNA2D4DepositorInsertCACNA2D4 (CACNA2D4 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Dendra2-Vimentin-C-18
Plasmid#57746PurposeLocalization: Intermediate Filaments, Excitation: 490 / 553, Emission: 507 / 573DepositorInsertVimentin (VIM Human)
UseTagsDendra2ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-human-BNIP3 delta Exon(2+3)
Plasmid#100783PurposeMammalian expression of human-BNIP3 delta Exon2+3 splice variantDepositorInserthuman-BNIP3 delta Exon(2+3) (BNIP3 Human)
UseTagsHAExpressionMammalianMutationExon2 and Exon3 deletionPromoterCMVAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDG_CACNG4_OE_1
Plasmid#161653PurposeOverexpresses CACNG4DepositorInsertCACNG4 (CACNG4 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos2-ARP2-C-14
Plasmid#57348PurposeLocalization: Actin Related Protein, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertARP2 (ACTR2 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABEpuro-H-RAS_V12
Plasmid#39526DepositorInsertHRAS V12 (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationV12 = G12VPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
mKO2-Rab7a-7
Plasmid#57893PurposeLocalization: Ras GTPase, Excitation: 551, Emission: 565DepositorInsertRab7a (RAB7A Human)
UseTagsmKO2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
mAmetrine-Actin-7
Plasmid#56541PurposeLocalization: Actin, Excitation: 406, Emission: 526DepositorInsertActin (ACTB Human)
UseTagsmAmetrineExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHBS996 TDP43 RRM-GFP A382T
Plasmid#107816PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationA382T mutation in LLPS reporterPromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
R777-E003 Hs.AKT2
Plasmid#70287PurposeGateway ORF clone of human AKT2 [NM_001626.5] with stop codon (for native or N-terminal fusions)DepositorInsertAKT 2 (AKT2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise-ATG13-C-18
Plasmid#55531PurposeLocalization: Membrane Receptor, Excitation: 434, Emission: 474DepositorInsertATG13 (ATG13 Human)
UseTagsmTurquoiseExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mOrange2-Gamma-Tubulin-17
Plasmid#57958PurposeLocalization: Centrosomes, Excitation: 549, Emission: 565DepositorInsertGamma-Tubulin (TUBG1 Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
hKv2.1-DN-HA
Plasmid#111530PurposeExpresses a non-conducting dominant neg in mammalian cellsDepositorInserthuman W369C-Y384T-Kv2.1 + HA (KCNB1 Human)
UseTagsHAExpressionMammalianMutationW369C-Y384TPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise-ATG14-N-18
Plasmid#55534PurposeLocalization: Membrane Receptor, Excitation: 434, Emission: 474DepositorInsertATG14 (ATG14 Human)
UseTagsmTurquoiseExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEXV3-Myc-H-RAS_V12
Plasmid#39528DepositorInsertHRAS V12 (HRAS Human)
UseEukaryoticTagsMycExpressionMutationV12 = G12VPromoterAvailable sinceNov. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
mRuby-FYN-N-10
Plasmid#55863PurposeLocalization: Membrane Associated Kinase, Excitation: 558, Emission: 605DepositorInsertFYN (FYN Human)
UseTagsmRubyExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMXD1.1.0-gDNA
Plasmid#112446PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MXD1DepositorInsertMXD1 (MXD1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXD1-donor
Plasmid#112350PurposeCRISPR donor plasmid to tag human transcription factor MXD1 with GFPDepositorInsertMXD1 homology arms flanking EGFP-IRES-Neo cassette (MXD1 Human)
UseCRISPRTagsExpressionMutationhg38:2:69937537:A>C,rs3771530PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFPKir7.1
Plasmid#107182PurposeAdenoassociatedvirus particle generationDepositorInsertKCNJ13 (KCNJ13 Human)
UseAAVTagsEGFPExpressionMutationPromoterCAGAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Alpha-Actinin2-N-18
Plasmid#57577PurposeLocalization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertAlpha-Actinin2 (ACTN2 Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mPA-GFP-Dectin1A-N-10
Plasmid#57129PurposeLocalization: Membrane, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertDectin1A (CLEC7A Human)
UseTagsmPA-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUSF2.1.0-gDNA
Plasmid#112457PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor USF2DepositorInsertUSF2 (USF2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUSF2-donor
Plasmid#112361PurposeCRISPR donor plasmid to tag human transcription factor USF2 with GFPDepositorInsertUSF2 homology arms flanking EGFP-IRES-Neo cassette (USF2 Human)
UseCRISPRTagsExpressionMutationhg38:19:35278400:T>A, hg38:19:35278455:G>CPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
5J39 (TDRKH)
Plasmid#79523PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTDRD2 (TDRKH Human)
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceJune 16, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits
