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DT034A
Plasmid#159395PurposeConstitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
UseSynthetic BiologyTagsFLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…ExpressionBacterialMutationPromoterJ23117 and pLux promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT050A
Plasmid#159411PurposeConstitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoterDepositorInsertpr.J23101-BDNF (Bdnf, Mouse) (Bdnf Synthetic, Mouse)
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterJ23101 promoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
phumanCas9 (GB0575)
Plasmid#68206PurposeProvides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid partDepositorInsertCas9 coding region (human codon optimised)
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removed; human codon optimis…PromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTNos:BASTA:PNos (GB0023)
Plasmid#68211PurposeProvides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.DepositorInsertbar gene
UseUnspecifiedTagsExpressionMutationBsaI and BsmBI sites removedPromoterPNosAvailable sinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pLacIBD::SDRX (GB0673)
Plasmid#68183PurposeProvides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertLacI Binding Domain fused to SRDX Transcriptional Repressor
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
LacI-pLacI-pI pm
Plasmid#218098PurposePhagemid that expresses LacI, and pI under the Lac promoterDepositorInsertpI
UseTagsExpressionBacterialMutationPromoterLac promoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
DT033A
Plasmid#159394PurposeConstitutive murine mature BDNF expression, controlled under E.coli tac promoterDepositorInsertpr.tac-BDNF (Bdnf, Mouse) (Bdnf Mouse)
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromotertac promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PatoL-LOG241
Plasmid#72846PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.DepositorInsertPatoL
UseTagsExpressionBacterialMutationPromoterPatoLAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMGF172
Plasmid#96998Purposeish1 mCherry insertion cassette with ura4+ geneDepositorInsertish1 (ish1 Fission Yeast)
UseTagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + ura4(…PromoterN/AAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLexA::SRDX (GB0732)
Plasmid#68184PurposeProvides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertLexA Binding Domain fused to SRDX Transcriptional Repressor
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD::BDR (GB0734)
Plasmid#68185PurposeProvides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertGal4 Binding Domain fused to BDR Transcriptional Repressor
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBOB-EF1-FastFUCCI-Puro
Plasmid#86849Purpose3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selectionDepositorInsertsmKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1 and PGKAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT-P2A-Clover-BlastR
Plasmid#138568PurposeUniversal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJDepositorInsertP2A-clover-BlastR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUPD P35S (GB0552)
Plasmid#160554Purpose35s promoter for N-terminal fusionsDepositorInsert35s
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJC5-4-insulator-BREreporter-insulator
Plasmid#171499Purposeinjections to make Bmp reporter mouse transgenic lineDepositorInsertInsulator BRE-MLP:H2B-Turqoise
UseTagsExpressionMammalianMutationPromoterBRE-MLPAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pISH-Ascl1
Plasmid#74338PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Ascl1 (also known as Mash1). Not for gene expression.DepositorInsertachaete-scute family bHLH transcription factor 1 (Ascl1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMGF157
Plasmid#96972Purposeish1-mCherry insertion cassette with Hygro resistance geneDepositorInsertish1 (ish1 Fission Yeast)
UseTagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + hygro…PromoterN/AAvailable sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only
