We narrowed to 18,217 results for: Met;

1. WT-Pan

   Plasmid

   #99638

   Purpose

   EGFP-mCherry control reporter for methionine misincorporation

   Depositor

   Tao Pan

   Article

   Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

   Insert

   mCherry
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pReporter_12

   Plasmid

   #60572

   Purpose

   A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Reporter_12
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Camui-CR

   Plasmid

   #40256

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Camuiα-CR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pclbw-mitoTagRFP

   Plasmid

   #58425

   Purpose

   Mammalian expression of tagRFP targeted to the mitochondrial matrix

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   mito-tagRFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cmv-bactin

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-AncMEcoGII291

   Plasmid

   #205024

   Purpose

   Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 291
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVPT-rtTR-KRAB-2SM2

   Plasmid

   #11652

   Purpose

   Tet-regulated (Tet-off) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   mPGK, GFP, rtTR-KRAB-2SM2, Tet-off
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

   Plasmid

   #84248

   Purpose

   Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgTRE3G
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. mOrange2-Lysosomes-20

   Plasmid

   #57968

   Purpose

   Localization: Lysosome Membrane, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   LAMP1
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNB769

   Plasmid

   #60138

   Purpose

   Red click beetle luciferase from Promega driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   CBR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available since

   Nov. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a-Core Traptavidin

    Plasmid

    #26054

    Depositor

    Mark Howarth

    Article

    Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

    Insert

    Core Traptavidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Streptavidin with 2 amino acid mutations: S52G R5…

    Promoter

    Available since

    Aug. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

11. M-NM-sgRNA

    Plasmid

    #48673

    Purpose

    Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. TS246_pNKI-2-13_HALO-DPN7

    Plasmid

    #200720

    Purpose

    producing Methyl Tracer HALO protein in bacteria

    Depositor

    Bas van Steensel

    Article

    van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

    Insert

    HALO-DPN7
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pG-HIV-2LTR

    Plasmid

    #104590

    Purpose

    standard for qPCR

    Depositor

    Kristine Yoder

    Article

    Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

    Insert

    2LTR circle junction of HIV-1
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

    Plasmid

    #84250

    Purpose

    Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sa sgTRE3G
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK392 (mAID-mClover-BSD)

    Plasmid

    #121193

    Purpose

    C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    mAID-mClover-BS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCVL SFFV-EF1s HA.NLS.Sce(opt)

    Plasmid

    #31479

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV/Ef1s HA NLS I-SceI
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    "opt" indicates the coding sequence has…

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-CyRFP1

    Plasmid

    #84356

    Purpose

    Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    CyRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ii-Str_SBP-EGFP-GPI

    Plasmid

    #65296

    Purpose

    synchronize trafficking of EGFP-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Use

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLI-TGD(1463000)

    Plasmid

    #85791

    Purpose

    P. falciparum integration plasmid to disrupt genes using SLI

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PF3D7146300 targeting region, GFP-2TA-Neo-R
    

    Use

    P. falciparum genomic integration (gene disruptio…

    Tags

    GFP

    Expression

    Mutation

    Promoter

    none

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Parp14-c011

    Plasmid

    #173098

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K791-F978

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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