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pHR-UCOE-EF1a-Zim3-dCas9-BFP

Plasmid

#188775

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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XRE-H2B-eGFP

Plasmid

#182294

Purpose

Fluorescent reporter for AhR activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

Xenobiotic Response Element (CYP1A1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

XRE

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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tetO-ASCL1-LMX1b-NURR1-PuroR

Plasmid

#182298

Purpose

Doxycycline-inducible vector expressing the ALN transcription factors and PuroR (all separated by 2A sequences)

Depositor

Kristen Brennand

Article

Powell et al Mol Psychiatry. 2021 Sep 7. pii: 10.1038

Insert

ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B Human)

Use

Lentiviral

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS395_PhCMV-SB100

Plasmid

#169633

Purpose

Expression vector encoding PhCMV driven SB100 sleeping beauty transposase (PhCMV-SB100-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven SB100

Expression

Mammalian

Promoter

PhCMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-LiLac

Plasmid

#184570

Purpose

Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV

Depositor

Gary Yellen

Article

Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

Has Service

AAV8

Insert

LiLac fluorescent sensor of lactate

Use

AAV

Tags

His7

Expression

Mammalian

Promoter

CAG

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-L

Plasmid

#182279

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pK18sB

Plasmid

#177838

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

Depositor

Gregg Beckham, Christopher Johnson

Article

Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

Type

Empty backbone

Use

Bacterial gene replacement

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5B

Plasmid

#172203

Purpose

Expresses KIF5B tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRPN265NB-LaG16-MNase

Plasmid

#170978

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Article

Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

Insert

Glutathione-S-Transferase/LaG16/MNase

Tags

GST

Expression

Bacterial

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-ChR2-YFP

Plasmid

#171622

Purpose

TRE-ChR2-YFP reporter

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

TRE-ChR2-YFP

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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