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Plasmid#80493Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expressionDepositorInsertGFPluc2
UseDonor vector (human)TagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZFN-AAVS1-KKR
Plasmid#159298PurposeZFN (right) targeting the AAVS1 locus with obligate heterodimer FokIDepositorInsertZFN targeting the AAVS1 locus
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-idCas9-vpr
Plasmid#89985Purposeinducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locusDepositorInsertdCas9-VPR
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-AAVS1_B3903c_attP
Plasmid#182144PurposeTo insert attP attachment site at AAVS1 in human cells via twinPEDepositorInsertAAVS1_B3903c_attP pegRNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-AAVS1_A3786c_attP
Plasmid#182143PurposeTo insert attP attachment site at AAVS1 in human cells via twinPEDepositorInsertA3786c-attP pegRNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE-SHH
Plasmid#134350PurposeTargeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locusDepositorInsertSHH (SHH Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-sgAAVS1-HepmCherry
Plasmid#192827PurposeExpresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mCherry from hepatocyte-specific promoterDepositorInsertmCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 for sgRNA and hepatocyte-specific promoter (HS…Available sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-35:AAVS1-mEGFP
Plasmid#91565PurposeHomology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFPDepositorInsertAAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 7, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-AAVS1-TALEN-L
Plasmid#159299PurposeTALEN (left) targeting the AAVS1 locus with obligate heterodimer FokIDepositorInsertTALEN targeting the AAVS1
UseTALENTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAVS1-TALEN-R
Plasmid#159300PurposeTALEN (right) targeting the AAVS1 locus with obligate heterodimer FokIDepositorInsertTALEN targeting the AAVS1
UseTALENTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only