1,931 results

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pJJS001

Plasmid

#188324

Purpose

PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Publication

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::GFP::Linker

Expression

Bacterial

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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L4440_BioBrick-hif-1-sgRNA-A

Plasmid

#177784

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Publication

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Expression

Bacterial

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGLOW79

Plasmid

#177339

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Publication

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mNeonGreen

Tags

mNeonGreen

Expression

Worm

Promoter

myo-3

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Plasmid

#159859

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2452 - [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159854

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Plasmid

#159849

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM255 - sgRNA mosTI Pmlc-2_GFP

Plasmid

#159835

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI Pmlc-2_GFP

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3823

Plasmid

#154123

Purpose

mec-4 promoter driving tet OFF tetR-L-QFAD in an RCME vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::tetR-L-QFAD::act-4 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3774

Plasmid

#154117

Purpose

tetO 7X enhancer with ∆mec-7 basal promoter driving GFP-C1

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

tetO 7X::∆mec-7p::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3732

Plasmid

#153086

Purpose

mec-4p GFP-C1 positive control for RMCE integration

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p GFP-C1 tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDD400

Plasmid

#137024

Purpose

SapTrap mNG-GLO donor

Depositor

Daniel Dickinson, Bob Goldstein

Insert

C. elegans GLO-optimized mNeonGreen

Use

CRISPR; Saptrap donor for cloning repair templat…

Tags

C. elegans GLO-optimized mNeonGreen

Expression

Worm

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDD346

Plasmid

#133311

Purpose

SapTrap mNG-C1 donor

Depositor

Daniel Dickinson

Insert

C. elegans codon-optimized mNeonGreen

Use

CRISPR; Saptrap donor for cloning repair templat…

Tags

C. elegans codon-optimized mNeonGreen

Expression

Worm

Promoter

None

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA_SZ1-RGG-GFP-RGG

Plasmid

#124932

Purpose

Expression of SZ1-RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

GFP

Expression

Mammalian

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET_MBP-RGG-RGG

Plasmid

#124946

Purpose

Expression of MBP-RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

MBP-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His and MBP

Expression

Bacterial

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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JH02

Plasmid

#73511

Purpose

mCherryo_PH pCFJ150 - contians C. elegans sequence optimized mCherry

Depositor

Bob Goldstein

Publication

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mCherryo::PH::tbb-2

Tags

mCherry

Promoter

pmex-5

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSR02

Plasmid

#69149

Purpose

Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

Depositor

Sander van den Heuvel

Publication

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

rps-27

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKPY197

Plasmid

#62599

Purpose

Encodes Thr412Gly-CePheRS Alpha Subunit

Depositor

David Tirrell

Publication

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr412Gly-CePheRS Alpha Subunit

Tags

HIS

Expression

Worm

Mutation

Thr412Gly

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJA55

Plasmid

#59935

Purpose

gRNA for cleavage near sqt-1(e1350) locus in C elegans

Depositor

Andrew Fire

Publication

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

sqt-1(e1350) gRNA2

Use

CRISPR

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCFJ1002

Plasmid

#44477

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, peel-1, [4-3] Gateway

Use

Gateway Cloning

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ1209

Plasmid

#44491

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR]

Use

Gateway expression clone

Available since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

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