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Showing: 441 - 480 of 549 results

  4. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  13. pCGMbX

    Plasmid
    #42300
    Depositor
    Steven Almo
    Type
    Empty backbone
    Use
    Tags
    BiP signal sequence, His6, and V5-epitope
    Expression
    Insect
    Mutation
    Promoter
    metallothonein
    Available since
    April 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    42300-plasmid-map-sequence-id-57104

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  28. pECIA14

    Plasmid
    #47051
    Purpose
    This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags
    Depositor
    Chris Garcia
    Article
    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.
    Type
    Empty backbone
    Use
    Secreted expression in drosophila culture
    Tags
    Alkaline Phosphatase (human, placental), Flag, HR…
    Expression
    Insect
    Mutation
    Promoter
    Metallothionein (Copper-Inducible)
    Available since
    Aug. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47051-plasmid-map-sequence-id-67169

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  29. pECIA2

    Plasmid
    #47032
    Purpose
    This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.
    Depositor
    Chris Garcia
    Article
    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.
    Type
    Empty backbone
    Use
    Secreted expression in drosophila culture
    Tags
    Fc(human IgG1), HRV 3C Protease Cleavage site, He…
    Expression
    Insect
    Mutation
    Promoter
    Metallothionein (Copper-Inducible)
    Available since
    Aug. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47032-plasmid-map-sequence-id-67069

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  31. GB4402

    Plasmid
    #215253
    Purpose
    Module for the expression of the N. nambi HISPS gene under the regulation of PMTB and TNOS and the constitutive expression of CPH,LUZ, H3H, EGFP and p19
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    SF-PMTB:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    215253-plasmid-map-sequence-id-460273

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  33. Khc73(1-623)-GFP

    Plasmid
    #31750
    Depositor
    Ron Vale
    Insert
    Khc73 (Khc-73 Fly)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Contains only aa 1-623, L466P
    Promoter
    Metallothionein
    Available since
    Nov. 9, 2011
    Availability
    Academic Institutions and Nonprofits only

  35. R777-E103 Hs.ICMT

    Plasmid
    #70387
    Purpose
    Gateway ORF clone of human ICMT [NM_012405.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ICMT (ICMT Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70387-plasmid-map-sequence-id-125836

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  38. R777-E104 Hs.ICMT-nostop

    Plasmid
    #70388
    Purpose
    Gateway ORF clone of human ICMT [NM_012405.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ICMT (ICMT Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70388-plasmid-map-sequence-id-125837

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  39. pHCL151

    Plasmid
    #134455
    Purpose
    TetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.
    Depositor
    Thomas Bernhardt
    Article
    Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111
    Insert
    linker-mScarlet-I for C-terminal fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    plac
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    134455-plasmid-map-sequence-id-270547

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  40. pHCL147

    Plasmid
    #134454
    Purpose
    TetR, lacI-Plac-ssdsbA-mscarC, Integrative plasmid for the expression of fully-secreted prey. C-terminal mScarlet-I fusion.
    Depositor
    Thomas Bernhardt
    Article
    Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111
    Insert
    linker-mScarlet-I for C-terminal fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    plac
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    134454-plasmid-map-sequence-id-270545

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Showing: 441 - 480 of 549 results