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Plasmid#192770PurposeExpresses NC-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG mcrBGA
Plasmid#192769PurposeExpresses NG-tagged MCRaeoBGA in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG mcrBCGA
Plasmid#192768PurposeExpresses NG-tagged MCRaeoBCGA in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG mcrBDGA
Plasmid#192767PurposeExpresses NG-tagged MCRaeoBDGA in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRmar-NG
Plasmid#192766PurposeExpresses NG-tagged MCRmar in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-CA
Plasmid#192765PurposeExpresses CA-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NB
Plasmid#192764PurposeExpresses NB-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG
Plasmid#192763PurposeExpresses NG-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2ExpressionMutationPromoterAvailable sinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAcBac1-Mb-sfGFP WT
Plasmid#197569PurposeExpression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
Pyl-tRNA (4 copies)
UseTagsV5-His6ExpressionMammalianMutationPromoterCMV and U6/H1Available sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-sfGFP 150TAG
Plasmid#197570PurposeExpression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP 150TAG
Pyl-tRNA (4 copies)
UseTagsV5-His6ExpressionMammalianMutationN150TAGPromoterCMV and U6/H1Available sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP CaDXMT
Plasmid#139165PurposeSB-transposon with constitutive bi-directional promoter. One side contains Coffee arabica dimethylxanthine methyl transferase (CaDXMT) ; the other side contains RFP and puromycin resistance genes.DepositorInsert3,7-dimethylxanthine N-methyltransferase 1 [Coffea arabica]
UseTransposonTagsExpressionMammalianMutationPromoterEF1αAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSBBi-RP CaMXMT
Plasmid#139172PurposeSB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica monomethylxanthine methyltransferase (CaMXMT); the other side contains RFP and puromycin resistance genes.DepositorInsertmonomethylxanthine methyltransferase 1 [Coffea arabica]
UseTransposonTagsExpressionMammalianMutationPromoterEF1αAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCfB13089
Plasmid#212676PurposeTEF promoterDepositorInsertTEF
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12706
Plasmid#212677PurposeEXP promoterDepositorInsertEXP
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12710
Plasmid#212680PurposeSyn8 terminatorDepositorInsertSyn8
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCfB12709
Plasmid#212681PurposeSynGuo terminatorDepositorInsertSynGuo
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB13014
Plasmid#212685Purposeintegration vector for single cassette with integration site E3DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12712
Plasmid#212683Purposeintegration vector for single cassette with integration site E2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12711
Plasmid#212682Purposeintegration vector for single cassette with integration site E2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB13015
Plasmid#212686Purposeintegration vector for single cassette with integration site E3DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB13016
Plasmid#212687Purposeintegration vector for single cassette with integration site E3DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12713
Plasmid#212684Purposeintegration vector for single cassette with integration site E2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-sfGFP-WT
Plasmid#212119PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)
UseTagsHis6ExpressionBacterialMutationPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHM6g.AQ_1489
Plasmid#11539DepositorInserttRNA methyltransferase
UseTagsHisTag-MBP, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCfB12717
Plasmid#212691Purposeintegration vector for three gene cassette with integration site C3DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12708
Plasmid#212678PurposeGPD promoterDepositorInsertGPD
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB13090
Plasmid#212679PurposeLip2 terminatorDepositorInsertLip2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12718
Plasmid#212692Purposeintegration vector for three gene cassette with integration site F3DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12715
Plasmid#212689Purposeintegration vector for three gene cassette with integration site E3DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB13017
Plasmid#212693Purposeintegration vector for three gene cassette with integration site of the gene 4HPPD locusDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12716
Plasmid#212690Purposeintegration vector for three gene cassette with integration site E4DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12714
Plasmid#212688Purposeintegration vector for three gene cassette with integration site E2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRC
Plasmid#192855PurposeCloning of promoter of interest in front of mkate2 reporter geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterno promoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pITC
Plasmid#192856PurposeCloning and expression of transcription factor gene under control of naringenin inducible promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPfdeARAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2a
Plasmid#105693PurposeBacterial expression plasmids for production of recombinant metallothionein 2aDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXLK_aFb
Plasmid#114230PurposePOC12368, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-bDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLK_dFq
Plasmid#114237PurposePOC12377, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type d-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLK_pFa
Plasmid#114229PurposePOC12367, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type p-aDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLC_pFa
Plasmid#114219PurposePOC12355, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type p-aDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLP_pFa
Plasmid#114239PurposePOC12379, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type p-aDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB11785
Plasmid#212699PurposegRNA targeting to the intergradtion site E4DepositorInsertgRNA targeting to the intergradtion site E4
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB10769
Plasmid#212694PurposegRNA targeting to the intergradtion site E2DepositorInsertgRNA targeting to the intergradtion site E2
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12719
Plasmid#212702PurposegRNA targeting to the intergradtion site F3DepositorInsertgRNA targeting to the intergradtion site F3
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB11787
Plasmid#212695PurposegRNA targeting to the intergradtion site E2DepositorInsertgRNA targeting to the intergradtion site E2
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB6637
Plasmid#212696PurposegRNA targeting to the intergradtion site E3DepositorInsertgRNA targeting to the intergradtion site E3
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8860
Plasmid#212697PurposegRNA targeting to the intergradtion site E3DepositorInsertgRNA targeting to the intergradtion site E3
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8856
Plasmid#212701PurposegRNA targeting to the intergradtion site C3DepositorInsertgRNA targeting to the intergradtion site C3
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8866
Plasmid#212703PurposegRNA targeting to the intergradtion site F3DepositorInsertgRNA targeting to the intergradtion site F3
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB6630
Plasmid#212700PurposegRNA targeting to the intergradtion site C3DepositorInsertgRNA targeting to the intergradtion site C3
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB6638
Plasmid#212698PurposegRNA targeting to the intergradtion site E4DepositorInsertgRNA targeting to the intergradtion site E4
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only