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Showing: 221 - 240 of 350 results

  3. pHPHS232

    Plasmid
    #228356
    Purpose
    Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.
    Inserts
    mTagBFP2
    Reverse tetracycline transactivator 3
    neo
    Use
    Tags
    Blasticidin S deaminase and iCasp9
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GV and gene trap
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228356-plasmid-map-sequence-id-455149

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  14. pLV/eGFP-U6-sgRNA

    Plasmid
    #170544
    Purpose
    A lentiviral backbone for homology directed insertion of eGFP. Containing only eGFP, flanked by restriction sites for insertion of homology arms and a sgRNA casstte to clone in sgRNA for HDR
    Depositor
    Jacob Giehm Mikkelsen
    Article
    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    170544-plasmid-map-sequence-id-377687

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  16. ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid
    #173208
    Purpose
    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1-T804N mScarlet-I-TOSI donor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK1
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    173208-plasmid-map-sequence-id-339517

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  17. ATP1A1_G3_Dual_sgRNA

    Plasmid
    #173202
    Purpose
    Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173202-plasmid-map-sequence-id-339519

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  18. AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

    Plasmid
    #184397
    Purpose
    Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    Firefly luciferase beta-globin-PTC39
    Use
    CRISPR; Donor plasmid for hdr
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    PTC in beta-globin at amino acid position 39
    Promoter
    Tet-On 3G
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184397-plasmid-map-sequence-id-362358

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  19. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

    Plasmid
    #184398
    Purpose
    Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    Renilla luciferase beta-globin-PTC39
    Use
    CRISPR; Donor plasmid for hdr
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    PTC in beta-globin at amino acid position 39
    Promoter
    Tet-On 3G
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184398-plasmid-map-sequence-id-362369

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  20. pLenti-BLRR

    Plasmid
    #158958
    Purpose
    Bioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).
    Depositor
    Charles P. Lai
    Article
    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589
    Insert
    BLRR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158958-plasmid-map-sequence-id-316701

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Showing: 221 - 240 of 350 results