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pREDIT_Cas9-MS2-BB_BbsI

Plasmid

#164802

Purpose

REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD_NES

Plasmid

#124706

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD_NES (RAB7A Human)

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pREDIT_Cas9n-MS2-BB_BbsI

Plasmid

#164804

Purpose

REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPE2iso3_Z

Plasmid

#148118

Purpose

Mammalian Expression of HshnRNPE2iso3

Depositor

Elisa Izaurralde

Insert

HshnRNPE2iso3 (PCBP2 Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation L239 to S compapred to th…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pZE31-DsRed-ms2x2

Plasmid

#26248

Depositor

Philippe Cluzel

Article

Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

Insert

ms2 binding sites (x2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2010

Availability

Academic Institutions and Nonprofits only

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epB_UbC_tdMS2cp-tagRFP-Tx2

Plasmid

#119908

Purpose

ePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

HA-tdMS2cp-tagRFP-Tx2-nls

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMS29: pDonor(PmcCAST)

Plasmid

#168162

Purpose

PmcCAST donor harboring non-functional cargo.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST right end (RE)
PmcCAST left end (LE)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112922

Purpose

Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pST39-Ubc13-MMS2

Plasmid

#184807

Purpose

Bacterial expression of yeast UEV-E2 complex

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

Ubc13, MMS2

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGH183_MS2-AIDΔDead-Hygro

Plasmid

#85414

Purpose

lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AIDΔDead and Hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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sg14x(MS2) MUC4.1

Plasmid

#101153

Purpose

gRNA with 14 MCP binding site

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pTET2-lucCP-3MS2

Plasmid

#198351

Purpose

Part of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTR

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Firefly Luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

pTET2

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hFluc-V5-6xbsMS2_U

Plasmid

#147639

Purpose

Mammalian Expression of 6xbsMS2

Depositor

Elisa Izaurralde

Insert

6xbsMS2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pORANGE Rims2-GFP KI

Plasmid

#131495

Purpose

Endogenous tagging of RIM2: C-terminal (amino acid position: S1551)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rims2, Rims2 Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_AA

Plasmid

#148255

Purpose

Mammalian Expression of HsNot4

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsUNR_AA

Plasmid

#148239

Purpose

Mammalian Expression of HsUNR

Depositor

Elisa Izaurralde

Insert

HsUNR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_T

Plasmid

#147556

Purpose

Mammalian Expression of HsSmg7

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7 (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutation G216A, A1521G and one non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsMex3B_AD

Plasmid

#148478

Purpose

Mammalian Expression of HsMex3B

Depositor

Elisa Izaurralde

Insert

HsMex3B (MEX3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pPlacPbuCas13b-gRNA-T-MS2

Plasmid

#184839

Purpose

Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119 and lac promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pscMS2(WTx2)-EGFP

Plasmid

#140258

Purpose

MS2CP-dependent translational repression

Depositor

Hirohide Saito

Article

Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

Insert

Two MS2CP-binding sites embedded in scaffold

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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