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Showing: 221 - 240 of 17278 results

  1. pSynSRE-Mut-T-Luc

    Plasmid
    #60490
    Purpose
    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988
    Depositor
    Timothy Osborne
    Article
    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.
    Insert
    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    Use
    Luciferase
    Tags
    Luciferase reporter
    Expression
    Mammalian
    Mutation
    Four point mutations (G->C or A->C) in the …
    Promoter
    Partial HMG-CoA synthase promoter (-324/-225) fus…
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60490-plasmid-map-sequence-id-332535

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  2. pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

    Plasmid
    #196678
    Purpose
    AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.
    Depositor
    Haining Zhong
    Article
    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:
    Insert
    PKIαmut-P2A-mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196678-plasmid-map-sequence-id-385123

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  5. pOpen-MMLV_RT (mut H)

    Plasmid
    #165546
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165546-plasmid-map-sequence-id-339423

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  10. pET30a-His6-LOXCATmut

    Plasmid
    #140085
    Purpose
    A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutation in H265A and R268A in LOX (used original…
    Promoter
    T7
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140085-plasmid-map-sequence-id-311165

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  12. pIRES(A7Mut)_CyOFP1_KCNQ1

    Plasmid
    #173162
    Purpose
    For mammalian expression of KCNQ1 and creating variants
    Depositor
    Al George
    Insert
    KCNQ1 (KCNQ1 Human)
    Use
    Tags
    IRES2(A7Mut)-CyOFP1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    173162-plasmid-map-sequence-id-342914

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  20. pAAV-hSyn-GRAB_OTmut

    Plasmid
    #185388
    Purpose
    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons
    Depositor
    Yulong Li
    Article
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has Service
    AAV1 and AAV9
    Insert
    GPCR activation based oxytocin control sensor GRAB_OTmut
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185388-plasmid-map-sequence-id-372581

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Showing: 221 - 240 of 17278 results