We narrowed to 7,477 results for: PAC

1. pMC037-HisMS2_PLP_pac

   Plasmid

   #128233

   Purpose

   Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

   Depositor

   Paul Freemont

   Article

   de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

   Inserts

   Maturation Protein
   Coat Protein Dimer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 15, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAc5.1B-EGFP

   Plasmid

   #21181

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

   Insert

   enhanced green flourescent protein
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. Epac-S-H187

   Plasmid

   #170348

   Purpose

   mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAcGFP-N1_CBP

   Plasmid

   #221903

   Purpose

   Transient expression of GFP-tagged CBP in mammalian cells

   Depositor

   Daniel Bose

   Insert

   CBP (CREBBP Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Epac-S-H188

   Plasmid

   #170349

   Purpose

   mT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   mT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCambia1300-PacI

   Plasmid

   #44183

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEYFP-N3-EPAC1

   Plasmid

   #113110

   Purpose

   Human EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).

   Depositor

   Xiaodong Cheng

   Article

   Mei et al J Biol Chem. 2002 Mar 29;277(13):11497-5

   Insert

   RapGEF3 (RAPGEF3 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   PCMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-Epac2

   Plasmid

   #118314

   Purpose

   Expression of mouse Epac2

   Depositor

   Philip Stork

   Article

   Liu et al Mol Cell Biol. 2008 Dec;28(23):7109-25.

   Insert

   Epac2 (Rapgef4 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX459V2.0-HypaCas9

   Plasmid

   #108294

   Purpose

   pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACBSR-hyg

    Plasmid

    #87830

    Purpose

    Expresses lambda-red system with hyg selection marker

    Depositor

    Pep Charusanti

    Article

    Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

    Insert

    hyg
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    None

    Promoter

    bla

    Available since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC8_MF (VE5596)

    Plasmid

    #139768

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    PH or p10

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACT2-Kre33

    Plasmid

    #99990

    Purpose

    Express yeast Kre33 with Y2H activation domain fusion

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Kre33 (KRE33 Budding Yeast)
    

    Use

    Tags

    Y2H activation domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. C1-MPAct-mCherry

    Plasmid

    #155222

    Purpose

    Monitor changes in the density of membrane-proximal F-actin (MPA)

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcGFP1-N1-Dsup

    Plasmid

    #90020

    Purpose

    To express GFP-tagged Dsup in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACYCDuet-1_Ec_rpoZ

    Plasmid

    #128837

    Purpose

    E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

    Depositor

    Seth Darst

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Insert

    rpoZ (rpoZ E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAC1801_pmax-dCasRx

    Plasmid

    #118634

    Purpose

    Transient transfection; Expresses dCasRx; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    dCasRx
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A

    Promoter

    CAGGS

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACYC-Skp-TF

    Plasmid

    #83921

    Purpose

    Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Skp
    Trigger Factor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pACYC-Taq

    Plasmid

    #102793

    Purpose

    Expresses Taq DNA polymerase under WT T7 promoter

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    Taq DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. C1-MPAct-mCitrine

    Plasmid

    #155220

    Purpose

    Monitor changes in the density of membrane-proximal F-actin (MPA)

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C1-MPAct-mRuby3

    Plasmid

    #155221

    Purpose

    Monitor changes in the density of membrane-proximal F-actin (MPA)

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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