We narrowed to 9,870 results for: SUB;

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pOP795

Plasmid

#235700

Purpose

Protein expression His-taged HsNRBF2_FL in bacteria

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

NRBF2 (NRBF2 Human)

Tags

His6(TEV)

Expression

Bacterial

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pOP796

Plasmid

#235701

Purpose

Protein expression of His-tagged HsNRBF2 MIT domain in bacteria

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

NRBF2 MIT domain (NRBF2 Human)

Tags

His6(TEV)

Expression

Bacterial

Mutation

aa 1-89

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX RBM42-HA

Plasmid

#229500

Purpose

Lentiviral expression vector to express RBM42 isoform 1 with HA-tag.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

RBM42 (RBM42 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-F-box:gs10:FRB:HA

Plasmid

#228531

Purpose

F-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

F-box:gs10:FRB:HA (SKP2 Human, Synthetic)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc:Rbx1:gs10:FRB:HA

Plasmid

#228534

Purpose

Human Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

myc:Rbx1:gs10:FRB:HA (RBX1 Human, Synthetic)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_ΔC2 (157)

Plasmid

#187720

Purpose

Express GST-tagged NEDD4L ΔC2 (157) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 157-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-156

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

Plasmid

#187721

Purpose

Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 349-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-348

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT

Plasmid

#187723

Purpose

Express His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 1-418+582-975 (NEDD4L Human)

Tags

His-TEV

Expression

Insect

Mutation

deletion of residues 419-581

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

Plasmid

#187708

Purpose

Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 381-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-380

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W3-W4-HECT (492)

Plasmid

#187709

Purpose

Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-491

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W4-HECT

Plasmid

#187710

Purpose

Express his-tagged NEDD4L W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 543-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-542

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_FL-CS

Plasmid

#187713

Purpose

Express the catalytically inactive GST-tagged NEDD4L FL in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL with C874 mutated to a serine (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

Cys874 mutated to a serine

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_W2-W3-W4-HECT (348)

Plasmid

#187716

Purpose

Express GST-tagged NEDD4L W2-W3-W4-HECT (348) in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues XX (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-347

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_WW34

Plasmid

#187718

Purpose

Express GST-tagged NEDD4L WW34 tandem domains in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-581 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

only residues 492-581

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC4 CD H976Y

Plasmid

#224250

Purpose

Expresses human KDAC4 (HDAC4) catalytic domain H976Y in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2017 Aug 29;56(34):4549-45

Insert

KDAC4 (HDAC4 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Only includes residues 648-1083 (catalytic domain…

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBacI-KDAC6 H216A

Plasmid

#224351

Purpose

Expresses human KDAC6 (HDAC6) H216A in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 216 mutated to alanine

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBacI-KDAC6 H611A

Plasmid

#224352

Purpose

Expresses human KDAC6 (HDAC6) H611A in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 611 mutated to alanine

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-CD28-mTFP1

Plasmid

#223638

Purpose

Lentiviral expression of human CD28 with mTFP1 tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

mTFP1

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-ALFA-CD28

Plasmid

#223630

Purpose

Lentiviral expression of human CD28 with ALFA tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

ALFA tag

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28(Y191F)

Plasmid

#223628

Purpose

Lentiviral expression of human CD28 (Y191F) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Mutation

Y191F

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28(Y209F)

Plasmid

#223627

Purpose

Lentiviral expression of human CD28 (Y209F) in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Mutation

Y209F

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28(CD45TM)

Plasmid

#223626

Purpose

Lentiviral expression of human CD28 (CD45TM) in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Mutation

CD28 transmembrane domain replaced by CD45 transm…

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28(Y191F)

Plasmid

#223625

Purpose

Lentiviral expression of human CD28 (Y191F) in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Mutation

Y191F

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD28(Y191F)

Plasmid

#223623

Purpose

Lentiviral expression of human CD28 (Y191F) with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Mutation

Y191F

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD28

Plasmid

#223622

Purpose

Lentiviral expression of human CD28 with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-PDL1-LgBiT

Plasmid

#223619

Purpose

Lentiviral expression of human PD-L1 with LgBiT tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

LgBiT

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28-LgBiT

Plasmid

#223616

Purpose

Lentiviral expression of human CD28 with LgBiT tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

LgBiT

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD86-SNAP

Plasmid

#223613

Purpose

Lentiviral expression of human CD86 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD80-SNAP

Plasmid

#223612

Purpose

Lentiviral expression of human CD80 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD28-CLIP

Plasmid

#223610

Purpose

Lentiviral expression of human CD28 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-CLIP-CD28

Plasmid

#223600

Purpose

Lentiviral expression of human CD28 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SSH-pSUMO-β′-COPI-WD40-Tyr33Ala

Plasmid

#208170

Purpose

Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Tyr33Ala substitution

Tags

His-tag, SUMO-tag, and Strep-tag

Expression

Bacterial

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM84

Plasmid

#220172

Purpose

CRISRPi guide RNA against BTF3

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

BTF3 (sgRNA) (BTF3 Human)

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pC1_oROS-HT_LF(C199S)

Plasmid

#216412

Purpose

Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT_LF(C199S)

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pC1-lifeact-oROS-HT

Plasmid

#216420

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

lifeact-oROS-HT

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMC00036 (aka pMC0223)

Plasmid

#212659

Purpose

Guide RNA targeting SERPINB3

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

SERBPINB3 guide RNA (SERPINB3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-LUC7L2_sgRNA1

Plasmid

#201602

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L2 (LUC7L2 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSynW SYT9 D197, 199, 330, 332N IRES GFP

Plasmid

#195703

Purpose

Lentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

full-length mouse SYT9 with D197, 199, 330, 332N …

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav2.2 BBS W391A pcDNA3

Plasmid

#206077

Purpose

Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.3R/MPL-shRNA4

Plasmid

#204529

Purpose

Lentiviral expression of MPL-shRNA4; gene knock down of human MPL

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-shRNA4 (MPL Human)

Use

Lentiviral

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 4761 - 4800 of 9870 results

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We narrowed to 9,870 results for: SUB;