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pCDM8 hCD81

Plasmid

#11588

Depositor

Shoshana Levy

Article

Oren et al Mol Cell Biol. 1990 Aug . 10(8):4007-15.

Insert

CD81 (CD81 Human)

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pMaster12

Plasmid

#58527

Purpose

Reprogram somatic cells to iPS cells

Depositor

Mario Capecchi

Article

Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

Inserts

neo
tk
oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 Human)
Myc (MYC Human)
EBNA-1
Amp
Lin28 (LIN28A Human)
NANOG (NANOG Human)
IRES
NR5A2 (NR5A2 Human)
miR302/367 (MIR367, MIR302A Human)

Expression

Mammalian

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLB2 CAG P2Gm

Plasmid

#19752

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Type

Empty backbone

Use

Lentiviral, Mouse Targeting, and RNAi

Expression

Mammalian

Available Since

Feb. 2, 2009

Availability

Academic Institutions and Nonprofits only

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pEF6-CSPG4-myc-his

Plasmid

#69037

Purpose

Expression of CSPG4 in mammalian cells

Depositor

Wensheng Wei

Article

Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10

Insert

CSPG4 (CSPG4 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

none

Promoter

EF-1 alpha

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-AtAFB2

Plasmid

#129715

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

no

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAGM47523

Plasmid

#153221

Purpose

Level 0 zCas9i vector

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

SpCas9 with introns and 2 NLS

Use

Synthetic Biology

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti HRE-Luc pGK Hygro

Plasmid

#118706

Purpose

reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

HRE (HIF-responsive promoter (3xHRE)-luc

Use

Lentiviral

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1 pegRNA2 with trimmed attP

Plasmid

#222347

Purpose

pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

AAVS1 attP-installation pegRNA2

Use

CRISPR

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AMPKAR

Plasmid

#192446

Purpose

Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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spMSP1D1

Plasmid

#173482

Purpose

Expresses spMSP1D1 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spMSP1D1

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP14

Plasmid

#141267

Purpose

Gateway-compatible Entry vector, with insert of NSP14 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pU6-sp-sgRNA-RNF2_+41nick

Plasmid

#135958

Purpose

S. pyogenes sgRNA for +41 nick in RNF2

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_+41 nicking sgRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pIMAY*

Plasmid

#121441

Purpose

Allelic exchange plasmid with PheS* counter-selection for Staphylococcus aureus

Depositor

Angelika Grundling

Article

Schuster et al Microbiology. 2019 Apr 3. doi: 10.1099/m

Type

Empty backbone

Use

Escherichia coli/staphylococcus aureus shuttle ve…

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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PB-SAM

Plasmid

#102559

Purpose

Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Inserts

MS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Mutation

D10A and N863A in Cas9 and N55K in MS2

Promoter

CAG and EF1A

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-SPI1

Plasmid

#97039

Purpose

Expresses SPI1 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Spi-1 Proto-Oncogene (SPI1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only
Lck-mTurquoise2

Plasmid

#98822

Purpose

In vivo visualization of the plasma membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lck

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-SF2

Plasmid

#99021

Purpose

Mammalian expression of SF2

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

SF2 (SRSF1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJYS1Ptac

Plasmid

#85545

Purpose

Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

Depositor

Sheng Yang

Article

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Inserts

Cpf1
recT

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Promoter

tac and unknown

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-C-CreintG

Plasmid

#69571

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Has Service

AAV1

Insert

kozak consensus - C-CreintG

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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Villinpromoter-blue-FlpOERT2

Plasmid

#67278

Purpose

4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

Depositor

Jannik Elverløv-Jakobsen

Article

Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

Insert

Villin-mTQ2-P2A-FlpO-ERT2

Tags

mTQ2, linked to FlpO through an P2A ribosomal ski…

Expression

Mammalian

Promoter

Villin

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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