We narrowed to 10,744 results for: AGA

1. pMSCV-FLAG-2xSTREP-CDKN1B-Puro

   Plasmid

   #172611

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDKN1B (CDKN1B Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-IP-EGFP-PA-BAX(ER)

   Plasmid

   #228941

   Purpose

   Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   BAX (BAX Human)
   Cyb5a (Cyb5a Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus and C-terminus are deleted. BAX(3-171)…

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

   Plasmid

   #211969

   Purpose

   Express gRNA against LIN28A with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting LIN28A (LIN28A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(LIN28A_5-3)-PGKpuroBFP-W

   Plasmid

   #211968

   Purpose

   Express gRNA against LIN28A with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting LIN28A (LIN28A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(JARID2_5-4)-PGKpuroBFP-W

   Plasmid

   #211966

   Purpose

   Express gRNA against JARID2 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting JARID2 (JARID2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-LIG1

   Plasmid

   #215131

   Purpose

   Expresses mCherry-LIG1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   LIG1 (LIG1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-APEX1

   Plasmid

   #215126

   Purpose

   Expresses mCherry-APEX1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   APEX1 (APEX1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-2xFLAG-2xSTREP-CCND1(P287A)-IRES-mCherry

   Plasmid

   #172637

   Purpose

   Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287A) and mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Pro287Ala

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-2xFLAG-2xSTREP-CDK6

   Plasmid

   #172614

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK6 (CDK6 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry

    Plasmid

    #172627

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p21-HA-NeoR

    Plasmid

    #215104

    Purpose

    Expresses p21-HA in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. FFSS-Cyclin D1-NeoR

    Plasmid

    #215087

    Purpose

    Expresses FFSS-Cyclin D1 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p21-HA(dPCNA)-NeoR

    Plasmid

    #215108

    Purpose

    Expresses p21-HA(dPCNA) in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    p21(dPCNA) denotes mutations M147A, D149A, F150A.

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro

    Plasmid

    #172618

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr283Ala

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-FLAG-2xSTREP-CCND3-Puro

    Plasmid

    #172619

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. sgTP53(B)

    Plasmid

    #85560

    Purpose

    Expresses sgRNA targeting human TP53 around codon F109

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    tumor protein p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pLVX-TetOne-FFSS-Cyclin D1 (T286A)

    Plasmid

    #215147

    Purpose

    Expresses FFSS-Cyclin D1 (T286A) in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Mutation

    With T286A mutation.

    Promoter

    TRE3GS

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-pegRNA-HEK3-CTTins-px330-scaffold

    Plasmid

    #180017

    Purpose

    Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT2-shATRX

    Plasmid

    #124258

    Purpose

    Expresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.

    Depositor

    Maria Castro

    Article

    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

    Insert

    shATRX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6-pegRNA-LRRK2-G2019S-3a

    Plasmid

    #180432

    Purpose

    Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    ncRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    LRRK2-G2019S

    Promoter

    U6

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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