We narrowed to 18,379 results for: Met

1. mTagRFP-T-Paxillin-22

   Plasmid

   #58024

   Purpose

   Localization: Focal Adhesions, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Paxillin
   

   Tags

   mTagRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pET28a-AncMEcoGII291

   Plasmid

   #205024

   Purpose

   Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 291
   

   Tags

   His6x tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCVL SFFV-EF1s HA.NLS.Sce(opt)

   Plasmid

   #31479

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   SFFV/Ef1s HA NLS I-SceI
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Mutation

   "opt" indicates the coding sequence has…

   Available Since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21a-Core Traptavidin

   Plasmid

   #26054

   Depositor

   Mark Howarth

   Article

   Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

   Insert

   Core Traptavidin
   

   Expression

   Bacterial

   Mutation

   Streptavidin with 2 amino acid mutations: S52G R5…

   Available Since

   Aug. 30, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pMK392 (mAID-mClover-BSD)

   Plasmid

   #121193

   Purpose

   C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   mAID-mClover-BS
   

   Use

   CRISPR

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pG-HIV-2LTR

   Plasmid

   #104590

   Purpose

   standard for qPCR

   Depositor

   Kristine Yoder

   Article

   Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

   Insert

   2LTR circle junction of HIV-1
   

   Use

   Cloning vector

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TS246_pNKI-2-13_HALO-DPN7

   Plasmid

   #200720

   Purpose

   producing Methyl Tracer HALO protein in bacteria

   Depositor

   Bas van Steensel

   Article

   van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

   Insert

   HALO-DPN7
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   codon optimized

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFRET-HSP33 cys

   Plasmid

   #16076

   Depositor

   Ulrich Hammerling

   Article

   Guzy et al Cell Metab. 2005 Jun;1(6):401-8. doi: 10

   Insert

   HSP33 cys
   

   Tags

   ECFP and EYFP

   Expression

   Mammalian

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

9. M-NM-sgRNA

   Plasmid

   #48673

   Purpose

   Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter
   

   Use

   CRISPR

   Promoter

   hU6

   Available Since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ii-Str_SBP-EGFP-GPI

    Plasmid

    #65296

    Purpose

    synchronize trafficking of EGFP-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-CyRFP1

    Plasmid

    #84356

    Purpose

    Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    CyRFP1
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pReporter_12

    Plasmid

    #60572

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_12
    

    Expression

    Bacterial

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTriEx-NLyn-Venus-Zdk1

    Plasmid

    #81042

    Purpose

    mammalian expression of plasma-membrane anchored Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Tags

    6x His tag, FLAG, NLyn, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSLI-TGD(1463000)

    Plasmid

    #85791

    Purpose

    P. falciparum integration plasmid to disrupt genes using SLI

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PF3D7146300 targeting region, GFP-2TA-Neo-R
    

    Use

    P. falciparum genomic integration (gene disruptio…

    Tags

    GFP

    Promoter

    none

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

    Plasmid

    #84248

    Purpose

    Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sp sgTRE3G
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Parp14-c011

    Plasmid

    #173098

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K791-F978

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

    Plasmid

    #84250

    Purpose

    Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sa sgTRE3G
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVPRT-tTR-KRAB

    Plasmid

    #11648

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hPrion, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TARG1-c002

    Plasmid

    #173108

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    TARG1
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    Full length protein

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSETa_ mEos4a

    Plasmid

    #51072

    Purpose

    Photoconverible Fluorescent Protein_ Fixative Resistant

    Depositor

    Loren Looger

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    mEos4a
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge