We narrowed to 18,217 results for: Met;

1. pFRET-HSP33 cys

   Plasmid

   #16076

   Depositor

   Ulrich Hammerling

   Article

   Guzy et al Cell Metab. 2005 Jun;1(6):401-8. doi: 10

   Insert

   HSP33 cys
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pTriEx-NLyn-Venus-Zdk1

   Plasmid

   #81042

   Purpose

   mammalian expression of plasma-membrane anchored Zdk1

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk1
   

   Use

   Tags

   6x His tag, FLAG, NLyn, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. TARG1-c002

   Plasmid

   #173108

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   TARG1
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   Full length protein

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRE-EGFP

   Plasmid

   #89871

   Purpose

   EGFP reporter vector for iTango system

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   tetO-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVPRT-tTR-KRAB

   Plasmid

   #11648

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hPrion, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP

   Plasmid

   #84251

   Purpose

   Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgCD95-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSETa_ mEos4a

   Plasmid

   #51072

   Purpose

   Photoconverible Fluorescent Protein_ Fixative Resistant

   Depositor

   Loren Looger

   Article

   Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   mEos4a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. CFP-tTA-ER-C3

   Plasmid

   #134569

   Purpose

   Expresses a CFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

   Depositor

   Susan Janicki

   Article

   Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

   Insert

   tTA-ER
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE30del

   Plasmid

   #106902

   Purpose

   pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ii-Str_neomycin

    Plasmid

    #65308

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Invariant chain (p33)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Streptavidin in its lumenal part

    Promoter

    CMV

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. paavCAG-pre-mGRASP-2A-dTomato

    Plasmid

    #51902

    Purpose

    pre-2Ato

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Insert

    presynaptic mGRASP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIntegrase_2

    Plasmid

    #60574

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Gi-c010

    Plasmid

    #173080

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GAP
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    mCitrine + last 10 residues of GNA1, codon optimi…

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. rArc

    Plasmid

    #178206

    Purpose

    Ratiometric version of the genetically encoded voltage indicator ArcLight-Q239.

    Depositor

    Stefan Heinemann

    Article

    Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

    Insert

    rArc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC118-FLIP-Puro

    Plasmid

    #84538

    Purpose

    a mock vector for generating FLIP targeting vectors

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    FLIP - Puro
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pUBC_HA_L7Ae-VP64

    Plasmid

    #68421

    Purpose

    Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    L7Ae
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Mutation

    Promoter

    human UbC

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-sgNeo2/Cre

    Plasmid

    #89652

    Purpose

    Expresses a Neomycin-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Neomycin
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-T3G-CERP2

    Plasmid

    #175498

    Purpose

    All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM6-mCherry

    Plasmid

    #66534

    Purpose

    Expresses mCherry under the control of the Concensus T7 Promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Consensus T7

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. VSVGwt-SBP-EGFP_puromycin

    Plasmid

    #65302

    Purpose

    VSVG reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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