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We narrowed to 14,168 results for: eng

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Showing: 2381 - 2400 of 14168 results

  1. pJZC48

    Plasmid
    #62336
    Purpose
    sgRNA + 1x COM with COM-VP64 effector for mammalian cells
    Depositor
    Wendell Lim, Stanley Qi
    Publication
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 1x COM binding module
    COM-VP64
    Use
    Lentiviral
    Tags
    VP64
    Expression
    Mammalian
    Mutation
    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA
    Promoter
    CMV and U6
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62336-plasmid-map-sequence-id-102259

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  2. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65779-plasmid-map-sequence-id-112660

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  6. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

    Plasmid
    #219565
    Purpose
    3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome
    Depositor
    Andrea Ventura
    Publication
    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586
    Insert
    HygroR_2A_H2B-GFP-N_loxP_mScarlet
    Use
    Unspecified
    Promoter
    EF-1a
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219565-plasmid-map-sequence-id-438331

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  7. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Publication
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    171625-plasmid-map-sequence-id-335505

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  8. pX330a dCas9-VP64

    Plasmid
    #92363
    Purpose
    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    dCas9-VP64
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    92363-plasmid-map-sequence-id-178232

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  10. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    175570-plasmid-map-sequence-id-344992

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  16. p28-2iDMD-puro

    Plasmid
    #88895
    Purpose
    28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.
    Depositor
    Michele Calos
    Publication
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    puromycin resistance enzyme
    mCherry
    Expression
    Mammalian
    Mutation
    codon optimized
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    88895-plasmid-map-sequence-id-451851

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  17. LentiGuide Cherry

    Plasmid
    #170510
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Pavel Tolar
    Publication
    Newman et al bioRxiv 2021.03.02.433398
    Inserts
    S. pyogenes sgRNA cassette
    mCherry
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    170510-plasmid-map-sequence-id-343694

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  19. pAAV-SynaptoTAG2

    Plasmid
    #175275
    Purpose
    AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.
    Depositor
    Wei Xu
    Publication
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    EGFP-Synaptobrevin-2; tdTomato (Vamp2 )
    Use
    AAV
    Tags
    EGFP and tdTomato (P2A cleavage)
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    175275-plasmid-map-sequence-id-349084

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Showing: 2381 - 2400 of 14168 results