We narrowed to 14,439 results for: eng
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Plasmid#108933PurposeSelflabeling tag at ATP SynthaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertATP synthase F1 subunit gamma (ATP5F1C Human)
TagsHalo7 tagExpressionMammalianPromoterCMVAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP
Plasmid#47904PurposeAn AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.DepositorInserteNpHR3.0
UseAAVTagsiRFPExpressionMammalianPromoterCaMKIIaAvailable sinceSept. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynaptoTAG2
Plasmid#175275PurposeAAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.DepositorInsertEGFP-Synaptobrevin-2; tdTomato (Vamp2 )
UseAAVTagsEGFP and tdTomato (P2A cleavage)PromoterSynapsinAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)
Plasmid#160736PurposeSingle-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-C-TEV 219
Plasmid#100096PurposeSNIPer plasmid, C terminal TEV fused to FKBPDepositorInsertC-TEV
UseRetroviralTagsFKBP and MycExpressionMammalianMutationC terminal domainPromoterCMVAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACS2
Plasmid#13747DepositorAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
Plasmid#158651Purposemaking transgenics expressing a UAS driven YFP and NTR 2.0DepositorInsert5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
UseTol2-based transgenic expression in zebrafish/oth…MutationF70A/F108YAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pN251
Plasmid#188471PurposeLentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)DepositorInsertenCas12a-HF (gift from Dr. Keith Joung)
UseLentiviralExpressionBacterial and MammalianAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR
Plasmid#126705Purposedonor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP
UseCRISPRAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFP
Plasmid#92388PurposeCell death reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertANNEXINV (ANXA5 Human)
UseZebrafish expressionTagsmVenusPromoterubbAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_MFE1_hrGFP
Plasmid#84617PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locusDepositorPublicationInserthrGFP
UseSynthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCM271rfp
Plasmid#59774PurposepCMrfp with TrfA mutation (271R to C)DepositorInsertRFP
ExpressionBacterialPromoterpBADAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-enAsCas12f
Plasmid#204637PurposeExpression of enAsCas12f in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertenAsCas12f
TagsNLSExpressionMammalianMutationD196K, N199K, G276R, N328G, D364RPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHen2 Avi-tag-linker-p3
Plasmid#119819PurposeFor cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophageDepositorInsertlinker with Avi-tag and p3
TagsAvi-tagExpressionBacterialAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL-p4X
Plasmid#75289PurposeExpresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding proteinDepositorTypeEmpty backboneTagsmaltose-binding proteinExpressionBacterialPromotertacAvailable sinceJuly 19, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-dCas9-MQ1(WT)-EGFP
Plasmid#89633PurposeTargeted CpG methylationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG and 6*HisExpressionMammalianPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
shHIF2α(#2) (HIF2α-#3-pSUPER-retro-GFP/Neo)
Plasmid#22132DepositorInsertsynthethic oligonucleotide
UseRNAi and RetroviralExpressionMammalianMutationsynthethic oligonucleotide encoding shRNA targeti…Available sinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA5/FRT/TO_H2B_HaloTag7
Plasmid#169329PurposeExpression of H2B-HaloTag7 in mammalian cellsDepositorAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJZC48
Plasmid#62336PurposesgRNA + 1x COM with COM-VP64 effector for mammalian cellsDepositorInsertssgRNA + 1x COM binding module
COM-VP64
UseLentiviralTagsVP64ExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only