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pLV-EF1a-ArgSen (-)P2A (-)NLS

Plasmid

#184699

Purpose

Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen (-)P2A (-)NLS

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET303-ColE1-T-P110A

Plasmid

#180237

Purpose

Expresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

Colicin E1

Tags

6x His-tag

Expression

Bacterial

Mutation

Truncation of ColE1 containing T domain of ColE1 …

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-G1pCNPRS

Plasmid

#174719

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-5-pyridyl)alanine (pCNP), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-FLAG-NLS-VP64-MCS_N-term

Plasmid

#136886

Purpose

Doxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

VP64 activator domain

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET30 W96A-GLTP

Plasmid

#170734

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

Insert

W96A-GLTP (GLTP Human)

Tags

His

Expression

Bacterial

Mutation

W96A

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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actc1b-TCAP-mKate2

Plasmid

#109496

Purpose

Muscle specific zebrafish expression of mKate2 fused to telethonin

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

TCAP (tcap Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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ORF18(R158A_L159A)-3xFLAG pCDNA4

Plasmid

#129814

Purpose

Expresses KSHV ORF18 (R158A_L159A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Arginine 158 to Alanine and Leucine 159 t…

Promoter

CMV

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(R74A_K75A)-3xFLAG pCDNA4

Plasmid

#129811

Purpose

Expresses KSHV ORF18 (R74A_K75A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Arginine 74 to Alanine and Lysine 75 to A…

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-hUCH37 (ISF1)(M148A/F149A)

Plasmid

#61933

Purpose

Expression of hUCH37 (M148A/F149A) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hUCH37 (ISF1) (UCHL5 Human)

Tags

6x HIS

Expression

Bacterial

Mutation

Methionine 148 and Phenylalanine 149 were mutated…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS_K36A_R38A_E39A

Plasmid

#65657

Purpose

FLAG-HA expression vector with RBPMS carrying mutation K36A, R38A, E39A

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

changed lysine 36 to alanine, arguing 38 to alani…

Promoter

CMV, 2x Tet

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SFFV L5-L2

Plasmid

#62098

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE-miR-28

Plasmid

#35686

Depositor

George Calin

Article

Almeida et al Gastroenterology. 2012 Jan 10.

Insert

hsa-miR-28 (MIR28 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pGSTag BimL(AAA)

Plasmid

#25063

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (AAA) (BCL2L11 Human)

Tags

Gst

Expression

Bacterial

Mutation

Changed serine 44 to alanine Changed threonine 5…

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (AAA)

Plasmid

#24244

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(AAA) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

changed Serine 44 to Alanine changed Threonine 5…

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf203

Plasmid

#12891

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf203

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf204

Plasmid

#12892

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf204

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf210

Plasmid

#12898

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf210

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf211

Plasmid

#12899

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf211

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf213

Plasmid

#12901

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf213

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf214

Plasmid

#12902

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf214

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only