We narrowed to 6,501 results for: nls
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Plasmid#183400PurposeDual expression of GI-VP64 and GAL4DBD-M13-SmBiT-FKF1 in the nucleusDepositorInsertGI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1
ExpressionMammalianPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107321PurposeExpresses SpCas9 (R1333S) fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1333S) is fused to SaCas9PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-NLRP3 711-1034
Plasmid#75141PurposeExpresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (Nlrp3 Mouse)
TagsFlagExpressionMammalianMutationfragment encoding amino acids 711-1034PromoterCMVAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-N-Flag-NLRP3 1-90
Plasmid#75137PurposeExpresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (Nlrp3 Mouse)
TagsFlagExpressionMammalianMutationfragment encoding amino acids 1-90PromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SOX9-T2A-NLS-tdTomato-PGK-Puro
Plasmid#89993PurposeDonor template for generation of SOX9-ntdTomato reporter cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
UseCRISPRPromoternoneAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9714: pHR-PGK-SV40_NLS-dCasMINI-V4-NZF-c-Myc_NLS-mCherry-WPRE
Plasmid#203220PurposeExpression of dCasMINI-V4-NZF-mCherry in mammalian cellsDepositorInsertdCasMINI-V4-NZF-mCherry
UseCRISPR and LentiviralExpressionMammalianPromoterPGKAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-T679A-NLS
Plasmid#118938PurposeUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
ExpressionPlantAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-NLRP3 91-710
Plasmid#75140PurposeExpresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (Nlrp3 Mouse)
TagsFlagExpressionMammalianMutationfragment encoding amino acids 91-710PromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTE_20 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-BARD1-NLS-HbaUTR
Plasmid#252016PurposeIn vitro transcription of Cas9-BARD1 TruEditor for mRNA generationDepositorPublicationInsertCas9-BARD1
UseCRISPRExpressionMammalianMutationNAAvailable sinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTE_22 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-SLX1A-NLS-HbaUTR
Plasmid#252018PurposeIn vitro transcription of Cas9-SLX1A TruEditor for mRNA generationDepositorPublicationInsertCas9-SLX1A
UseCRISPRExpressionMammalianMutationNAAvailable sinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pET42b-AncBE4max
Plasmid#165157PurposeExpresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expressionDepositorInsertAncBE4max
Tags6xHisExpressionBacterialAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMPE-ePPEplus
Plasmid#205242PurposeFor multiplex plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCsy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA
Plasmid#197024PurposeVector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized OsCas12f1
ExpressionMammalianPromoterCBh, CMV, hU6Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS
Plasmid#112215PurposeTargeted DNA methylationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseCRISPRTagsmCherryMutationD10A,H840A,N863A,C710SAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268
Plasmid#69224PurposeExpresses R1335K mutant SpCas9 fused to Zif268 in mammalian cellDepositorInsertSpCas9
UseCRISPRTagsNLS-3XHA-NLS and Zif268ExpressionMammalianMutationArginine 1335 is changed to LysineAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCM61
Plasmid#261133PurposeFor N-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression. No promoter. Can be integrated at single-copy into genome using RMCE.DepositorPublicationInsertAID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC
ExpressionWormPromoternoneAvailable sinceSept. 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCM60
Plasmid#261132PurposeFor N-terminal tagging a protein of interest with VC155 (split-YFP) and mCherry expression. No promoter. Can be integrated at single-copy into genome using RMCE.DepositorPublicationInsertAID::nls::mcherry::2a::AID::VC155::tbb-2 3' in pLF3FShC
ExpressionWormPromoternoneAvailable sinceSept. 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCM62
Plasmid#261130PurposeFor C-terminal tagging a protein of interest with VC155 (split-YFP) and mCherry expression. No promoter. Can be integrated at single-copy into genome using RMCE.DepositorPublicationInsertAID::nls::mcherry::2a::AID::VC155::tbb-2 3' in pLF3FShC
ExpressionWormPromoternoneAvailable sinceSept. 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCM63
Plasmid#261131PurposeFor C-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression. No promoter. Can be integrated at single-copy into genome using RMCE.DepositorPublicationInsertAID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC
ExpressionWormPromoternoneAvailable sinceAug. 31, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEB2_4_CusR-VIII
Plasmid#236219PurposeCusR-based expression system: Plasmid with cusR-mTurquoise2-VP64-SV40 under RET2p and mCherry under 8xCusR_BSsENO1cp with homologous regions for genome integration into HO locus.DepositorInsertscusR
mCherry, SSA1 terminator
UseSynthetic BiologyTagsmTurquoise2-VP64-NLSExpressionBacterialPromoter8xCusR_BSsENO1cp and RET2pAvailable sinceAug. 18, 2026AvailabilityAcademic Institutions and Nonprofits only