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pMXs-hs-HRAS

Plasmid

#52730

Purpose

retroviral expression of human HRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

HRAS (HRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pPHR-iRFP713-NLS-VP16

Plasmid

#103823

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-iRFP713-VP16 (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-NLS-VP16

Plasmid

#103821

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-mCherry-VP16 (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-hNCL

Plasmid

#103805

Purpose

BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hNCL (CIB1, NCL Human, Mustard Weed)

Expression

Mammalian

Mutation

hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-TetR-tagRFP-T

Plasmid

#103809

Purpose

'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-TetR-tagRFP-T (CIB1, tetR E. coli, Mustard Weed)

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hNCL

Plasmid

#103806

Purpose

BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hNCL (CIB1, NCL Human, Mustard Weed)

Expression

Mammalian

Mutation

hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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p53 S23A targeting vector

Plasmid

#12167

Depositor

Tyler Jacks

Article

MacPherson et al EMBO J. 2004 Sep 15. 23(18):3689-99.

Insert

p53 (Trp53 Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

Ser23 mutation (S23A) in exon 2 and puromycin gen…

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pPHR-EYFP-NLS-VP16

Plasmid

#103822

Purpose

Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-VP16 (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

VP16: G126T (silent, G42G)

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAP-Tag5-IgKsp-mSLF-Myc-His

Plasmid

#249175

Purpose

Overexpression of C-terminal fragment of mouse Sparcl1 with Myc and 6xHis tag

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Tags

6xHis and Myc

Expression

Mammalian

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-mSLF-Myc-His

Plasmid

#249179

Purpose

AAV mediated overexpression of C-terminal fragment of mouse Sparcl1

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Use

AAV

Tags

6xHis and Myc

Expression

Mammalian

Mutation

C-terminal fragment

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-mHevin-Myc-His

Plasmid

#249178

Purpose

AAV mediated overexpression of full length mouse Sparcl1

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Use

AAV

Tags

6xHis and Myc

Expression

Mammalian

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pAP-Tag5-IgKsp-DE-Myc-His

Plasmid

#249176

Purpose

Overexpression of mouse Sparcl1 fragment a.a. 330-550 with Myc and 6xHis tag

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

amino acids 330–550

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pPD685 HBB_IVS2-EnAsCas12a-BFP

Plasmid

#249158

Purpose

Overexpression of EnAsCas12a-BFP with HBB IVS2 intron in 5' UTR with blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

HBB_IVS2-EnAsCas12a-TagBFP (HBB Human, Synthetic)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

HBB IVS2 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSA099 PGK1_IVS3-Cas9-BFP

Plasmid

#249149

Purpose

Overexpression of SpCas9-BFP with PGK1 IVS3 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

PGK1_IVS3-Cas9-TagBFP (PGK1 S. pyogenes Cas9, synthetic, Human)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

PGK1 IVS3 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSA100 RPS12_IVS1-Cas9-BFP

Plasmid

#249150

Purpose

Overexpression of SpCas9-BFP with RPS12 IVS1 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

RPS12_IVS1-Cas9-TagBFP (RPS12 S. pyogenes Cas9, synthetic, Human)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

RPS12 IVS1 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSA093 PFDN2_IVS3-Cas9-BFP

Plasmid

#249143

Purpose

Overexpression of SpCas9-BFP with PFDN2 IVS3 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

PFDN2_IVS3-Cas9-TagBFP (PFDN2 S. pyogenes Cas9, synthetic, Human)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

PFDN2 IVS3 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSA098 RPL18A_IVS4-Cas9-BFP

Plasmid

#249148

Purpose

Overexpression of SpCas9-BFP with RPL18A IVS4 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

RPL18A_IVS4-Cas9-TagBFP (RPL18A S. pyogenes Cas9, synthetic, Human)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

RPL18A IVS4 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pAP-Tag5-IgKsp-mHevin-Myc-His

Plasmid

#249174

Purpose

Overexpression of full length mouse Sparcl1 with Myc and 6xHis tag

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Tags

6xHis and Myc

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-shMfn1-mCherry-CAAX

Plasmid

#228740

Purpose

Membrane-tethered mCherry shRNA targeting Mfn1

Depositor

Cagla Eroglu

Article

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Mfn1 (Mfn1, Mfn1 Mouse, Rat)

Expression

Mammalian

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-bio-myc-mCE 211-597

Plasmid

#112716

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 211-597 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

deleted amino acids 2-210 (triphosphatase domain)

Promoter

CMV

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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