163,606 results

1. TDP-REGv2 Triple Cryptic Cre recombinase in pTwist-CMV

   Plasmid

   #216162

   Purpose

   Expresses Cre recombinase in cells lacking TDP-43 function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   Cre recombinase with N-terminal SV40 NLS and C-terminal c-Myc NLS
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnCas9-AID

   Plasmid

   #196242

   Purpose

   Plasmid expressing the nCas9-AID fusion

   Depositor

   Andrea Puhar

   Article

   Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

   Insert

   nickase Cas9 fused to AID
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x

   Plasmid

   #182256

   Purpose

   Human PDAP1 3` UTR region mutated in 4x binding site for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PDAP1 gene mutated in 4 binding sites for mir-150
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SLP2a-TIR-GFP

   Plasmid

   #52738

   Purpose

   Retroviral vector expresses the lipid binding domain of SLP2a fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker

   Depositor

   Jonathan Kagan

   Article

   Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

   Insert

   Toll/IL-1 Receptor adaptor protein (TIRAP Human)
   

   Use

   Retroviral

   Tags

   C2A domain of Murine SLP2a and GFP

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-85

   Promoter

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pB-CAGGS-dCas9-KRAB

   Plasmid

   #110822

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CAGGS

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPRv2 hygro

   Plasmid

   #98291

   Purpose

   Variant of lentiCRISPRv2 that confers hygromycin resistance

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. RFP-LC3B

   Plasmid

   #200943

   Purpose

   RFP fused with LC3B for reporting LC3B lipidation

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

   Plasmid

   #85165

   Purpose

   Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.

   Depositor

   Robin Dowell

   Article

   Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

   Insert

   EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
   

   Use

   Tags

   AmilCP and RTX

   Expression

   Bacterial

   Mutation

   Promoter

   J23100 promoter

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-BbsI-PITCh

   Plasmid

   #127875

   Purpose

   For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-miniTurbo-NLS-Blast

    Plasmid

    #232347

    Purpose

    Lentiviral control vector expressing miniTurboNLS without dCas9

    Depositor

    Ali Shilatifard

    Article

    Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

    Insert

    miniTurbo
    

    Use

    Lentiviral

    Tags

    3xHA and 3xSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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