We narrowed to 4,331 results for: ARA-2

1. zgc:162148_R (OZ580)

   Plasmid

   #35220

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting zgc:162148 (micu1 Zebrafish)
   

   Use

   Zebrafish targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2012

   Availability

   Academic Institutions and Nonprofits only

2. CL20_mEGFP_TRIOBP_RBFOX2

   Plasmid

   #205947

   Purpose

   Express mEGFP-tagged fusion protein, TRIOBP_RBFOX2 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   TRIOBP_RBFOX2_L (TRIOBP, RBFOX2 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. zgc:162148_L (OZ579)

   Plasmid

   #35219

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting zgc:162148 (micu1 Zebrafish)
   

   Use

   Zebrafish targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

4. PM2-FRB-CFP

   Plasmid

   #67518

   Purpose

   FRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   CFP, HA, and PM tethering sequence from human Lyn…

   Expression

   Mammalian

   Mutation

   FRB domain only (residues 2019–2114)

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

   Plasmid

   #107857

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 1) (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMH-HT_SsCHAD-H29A-R32A-R36A-R69A

   Plasmid

   #127787

   Purpose

   Expresses a SsCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   mutations in His29, Arg32, Arg36 and Arg69 to Ala

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCIFR-5

   Plasmid

   #233294

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   p14G-BCD2

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1-EGFP-Aubergine 4R-K (R11K/R13K/R15K/R17K) (HK121)

   Plasmid

   #206433

   Purpose

   Aubergine expression in Schneider cells for imaging

   Depositor

   Mandy Jeske

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   Aubergine (aub Fly)
   

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   R11K/R13K/R15K/R17K

   Promoter

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1-mCherry-Bruno isoform A (JM78)

   Plasmid

   #206446

   Purpose

   Bruno expression in Schneider cells for imaging

   Depositor

   Mandy Jeske

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   Bruno (aret Fly)
   

   Use

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1-PH-mEGFP-Bruno isoform A (JM165)

    Plasmid

    #206452

    Purpose

    Bruno expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Bruno (aret Fly)
    

    Use

    Tags

    PH mEGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1-PH-mCherry-Bruno isoform A (HK72)

    Plasmid

    #206471

    Purpose

    Bruno expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Bruno (aret Fly)
    

    Use

    Tags

    PH mcherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHBS1507 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-S]

    Plasmid

    #133331

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All VLIM to S in CTD of full-length TDP43

    Promoter

    Available since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHBS1508 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-F]

    Plasmid

    #133332

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All VLIM to F in CTD of full-length TDP43

    Promoter

    Available since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMH-HT_CtCHAD-H253A-R256A-R260A

    Plasmid

    #127788

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A

    Plasmid

    #127789

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMH-HT_CtCHAD-R385A-K386A-K389A-K390A

    Plasmid

    #127792

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in Arg3…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHBS1399 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W+G385W]

    Plasmid

    #118813

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G368W and W385G in CTD of full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHBS1401 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

    Plasmid

    #118808

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All F to S in CTD of full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHBS1404 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-S]

    Plasmid

    #118809

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All VLIM to S in CTD of full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHBS1405 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-F]

    Plasmid

    #118810

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All VLIM to F in CTD of full-length TDP43

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBAD_GFP11_T7LysH17A

    Plasmid

    #59591

    Purpose

    Expression vector for solubility screening of proteins or protein fragments using a split-GFP assay.

    Depositor

    Richard Kingston

    Article

    Bulloch et al Anal Biochem. 2014 Jul 10. pii: S0003-26

    Type

    Empty backbone

    Use

    Tags

    6xHis-tag and GFP11

    Expression

    Bacterial

    Mutation

    Promoter

    pARA

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJRK254

    Plasmid

    #173742

    Purpose

    donor plasmid for SapTrap assembly

    Depositor

    Mike Boxem

    Article

    Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

    Insert

    VHH(mCherry)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHSG-PCNA2

    Plasmid

    #66126

    Purpose

    S. solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Chembiochem. 2010 Jul 26;11(11):1517-20.

    Insert

    DNA polymerase sliding clamp 2 (SSO_RS05185 Sulfolobus solfataricus P2)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LentiU6-mNeurog2 gRNA_1-MS2-Puro

    Plasmid

    #192680

    Purpose

    Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTKA001

    Plasmid

    #164577

    Purpose

    Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp7 (ORF1ab )
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. lgp120+linker-pEGFP N1

    Plasmid

    #62963

    Purpose

    rat lgp120 + 10 amino acid linker cloned into pEGFP N1. The linker separates the targetting motif in lgp120 from EGFP.

    Depositor

    Paul Luzio

    Insert

    lgp120 (Lamp1 Rat)
    

    Use

    Tags

    EGFP and linker

    Expression

    Mammalian

    Mutation

    has 2 amino acid mutations compared to the coding…

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pTatAkBxCs

    Plasmid

    #178544

    Purpose

    pTatABC with KpnI, XbaI, and KpnI restriction sites after TatA, TatB, and TatC, respectively.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    TatA, TatB, TatC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araC

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD_TatABmCherryC

    Plasmid

    #178545

    Purpose

    pTatABC with an mCherry fluorescent protein tag at the end of TatB.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    TatA, TatBmCherry, TatC
    

    Use

    Tags

    mCherry fused to TatB

    Expression

    Bacterial

    Mutation

    Promoter

    araC

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PB-TO-ASCL1-DLX2

    Plasmid

    #182307

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and pTRE3G-bidirectional

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. BBOX1

    Plasmid

    #39176

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BBOX1 (BBOX1 Human)
    

    Use

    Baculovirus expression

    Tags

    His Tev

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only