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Showing: 4801 - 4840 of 8780 results

  1. pBHM2643

    Plasmid
    #229527
    Purpose
    Carries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mAID_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    229527-plasmid-map-sequence-id-459548

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  2. 1203F-5’+3'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid
    #221536
    Purpose
    EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' and 3' end, 22nt mCherry-targeting spacer in between DRs
    Depositor
    Omar Akbari
    Insert
    eGFP
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221536-plasmid-map-sequence-id-437446

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  5. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Tags
    His-tag
    Expression
    Bacterial
    Available Since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164898-plasmid-map-sequence-id-327989

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  6. YARS2_pNIC-CTB10H

    Plasmid
    #153060
    Purpose
    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase 2
    Depositor
    Susanne Gräslund
    Insert
    YARS2 (YARS2 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153060-plasmid-map-sequence-id-304480

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  7. pENTRY4_ Dja6_2

    Plasmid
    #126895
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126895-plasmid-map-sequence-id-250173

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  8. pENTRY4_ Rac4

    Plasmid
    #126896
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126896-plasmid-map-sequence-id-250171

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  9. pENTRY4_ CUL3

    Plasmid
    #126903
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126903-plasmid-map-sequence-id-250107

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  10. pENTRY4_ WRKY28_1

    Plasmid
    #126899
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    126899-plasmid-map-sequence-id-250041

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  11. pENTRY4_ WRKY28_2

    Plasmid
    #126900
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    126900-plasmid-map-sequence-id-249965

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  12. pENTRY4_ PLDbeta1

    Plasmid
    #126902
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126902-plasmid-map-sequence-id-249806

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  13. pENTRY4_ Dja6_1

    Plasmid
    #126894
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126894-plasmid-map-sequence-id-249699

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  14. pCASCADE-gapAP1

    Plasmid
    #87146
    Purpose
    gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87146-plasmid-map-sequence-id-174938

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  15. pGEX-6p1-GST-Ubiquitin

    Plasmid
    #223236
    Purpose
    Bacterial expression construct for GST-Tagged Ubiquitin production and purification
    Depositor
    Weixing Zhao
    Insert
    Ubiquitin
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    223236-plasmid-map-sequence-id-443802

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  16. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

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  17. xlox(GFP)hTERT

    Plasmid
    #69809
    Purpose
    MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a GFP marker
    Depositor
    David Ott
    Insert
    hTERT Reverse Transcriptase (TERT )
    Use
    Retroviral
    Tags
    none
    Promoter
    MLV promoter
    Available Since
    Oct. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    69809-plasmid-map-sequence-id-127377

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  18. pZP06C

    Plasmid
    #232322
    Purpose
    pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.
    Depositor
    Chenggang Wu
    Insert
    mCherry
    Expression
    Bacterial
    Promoter
    rpsJ
    Available Since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232322-plasmid-map-sequence-id-462278

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  19. Sperm whale Mb 64Y68F in pVP80K

    Plasmid
    #86956
    Purpose
    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).
    Depositor
    John Olson
    Insert
    Mb 64Y68F
    Expression
    Bacterial
    Mutation
    64Y68F
    Promoter
    T5
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    86956-plasmid-map-sequence-id-176792

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  20. pZP06

    Plasmid
    #232320
    Purpose
    pZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232320-plasmid-map-sequence-id-462279

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  21. pcDNA3+ hSlo2.2

    Plasmid
    #16214
    Depositor
    Larry Salkoff
    Insert
    KCNT1 potassium channel, subfamily (KCNT1 Human)
    Expression
    Mammalian
    Mutation
    This construct lacks the first 53aa when compared…
    Available Since
    Jan. 18, 2008
    Availability
    Academic Institutions and Nonprofits only
    16214-plasmid-map-sequence-id-248256

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  22. pKT-mPDGFb-IRES-Katushka

    Plasmid
    #133082
    Purpose
    Expresses mouse PDGFb and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    PDGFb (Pdgfb Mouse)
    Expression
    Mammalian
    Available Since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133082-plasmid-map-sequence-id-261113

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  23. pBHM2647

    Plasmid
    #229989
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229989-plasmid-map-sequence-id-461139

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  24. pBHM2648

    Plasmid
    #229990
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229990-plasmid-map-sequence-id-461146

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  25. pBHM2649

    Plasmid
    #229991
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229991-plasmid-map-sequence-id-461155

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  26. pET22B_PcrA_2Bchimera

    Plasmid
    #102998
    Purpose
    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA
    Depositor
    Martin Webb
    Insert
    PcrA_2Bchimera
    Expression
    Bacterial
    Mutation
    Bacilllus stearothermophilus PcrA in which the 2B…
    Promoter
    T7
    Available Since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102998-plasmid-map-sequence-id-199442

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  27. PsbA2-HMGS-HMGR-ATOB-NPTI

    Plasmid
    #73338
    Purpose
    Construct expressing the upper MVA pathway in Synechocystis PCC 6803
    Depositor
    Anastasios Melis
    Insert
    HMGS-HMGR-ATOB-NPTI
    Promoter
    psbA2
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73338-plasmid-map-sequence-id-135049

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  28. pOX + hSlo2.2

    Plasmid
    #16215
    Depositor
    Larry Salkoff
    Insert
    KCNT1 potassium channel, subfamily (KCNT1 Human)
    Use
    Xenopus oocyte expression
    Mutation
    This construct lacks the first 53aa when compared…
    Available Since
    Jan. 18, 2008
    Availability
    Academic Institutions and Nonprofits only

  29. pGGG-TaBSS2-TaSEP1B6(int)

    Plasmid
    #242016
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic SEP1-B6 driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaSEP1-B6_with_introns
    Tags
    3x FLAG
    Expression
    Plant
    Promoter
    BSS2
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242016-plasmid-map-sequence-id-504530

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  30. pGGG-TaBSS2-TaMOFB1(int)

    Plasmid
    #242015
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic MOF-B1 driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaMOF-B1_with_introns
    Tags
    3x FLAG
    Expression
    Plant
    Promoter
    BSS2
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242015-plasmid-map-sequence-id-504508

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  31. pBrg1-GGSG_AID_3xF_PP Target2

    Plasmid
    #235523
    Purpose
    Targeting construct to make C-term fusion with GGSG-AID-3xFLAG, roxed PgkPuro
    Depositor
    Brian Hendrich
    Insert
    Smarca4 (Smarca4 Mouse)
    Use
    Mouse Targeting
    Tags
    AID, 3xFLAG
    Mutation
    None
    Promoter
    PGK
    Available Since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    235523-plasmid-map-sequence-id-474040

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  32. pZP05

    Plasmid
    #232319
    Purpose
    pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum
    Depositor
    Chenggang Wu
    Insert
    Ori-repA
    Expression
    Bacterial
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232319-plasmid-map-sequence-id-462197

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  33. L1PB1 - pSPCH--PhiC31-tUBQ1 - pMUTE--Bxb1-tUBQ10

    Plasmid
    #219699
    Purpose
    Level 1 PhiC31 and Bxb1 integrase construct for tracking of stomata development: pSPCH promoter for PhiC31 and pMUTE promoter for Bxb1, no tag
    Depositor
    Jennifer Nemhauser
    Insert
    pSPCH::PhiC31:tUBQ1 - pMUTE::Bxb1:tUBQ10
    Expression
    Plant
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219699-plasmid-map-sequence-id-435730

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  34. pRS415 GPDpro-RNQ1-CFP

    Plasmid
    #224887
    Purpose
    Low copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).
    Depositor
    Paul Herman
    Insert
    RNQ1 (RNQ1 Budding Yeast)
    Tags
    CFP
    Expression
    Yeast
    Promoter
    GPD
    Available Since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    224887-plasmid-map-sequence-id-448181

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  35. pANY4-pR18/pL18-ccdB

    Plasmid
    #164899
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Tags
    His-tag
    Expression
    Bacterial
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164899-plasmid-map-sequence-id-327990

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  36. pCASCADE-udhA-zwf

    Plasmid
    #87153
    Purpose
    udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    udhA-zwf guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87153-plasmid-map-sequence-id-174933

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  40. single polypeptide FPX biosensor for caspase-3

    Plasmid
    #60883
    Purpose
    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    single polypeptide FPX biosensor for caspase-3
    Tags
    HindIII site and NES sequence LALKLAGLDIGS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60883-plasmid-map-sequence-id-150659

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Showing: 4801 - 4840 of 8780 results