We narrowed to 13,459 results for: 109

1. pCMV7.1_3xFLAG-LATS1

   Plasmid

   #172986

   Purpose

   constitutive expression of FLAG-tagged LATS1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-LATS1 (LATS1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiDP1.2

   Plasmid

   #171095

   Purpose

   lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   OsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Emerald, Geminin, miniAID, mCherry2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PDI-QUAD-V5-pcDNA3.1

   Plasmid

   #153550

   Purpose

   PDI-QUAD was generated from PDI-WT pcDNA3.1 (+) using two native active sites (CGHC, CGHC) tagged with V5, and mutated (to SGHS, SGHS) using site directed mutagenesis.

   Depositor

   Julie Atkin

   Article

   Parakh et al iScience. 2020 May 22;23(5):101097. doi:

   Insert

   Protein disulphide isomerase
   

   Tags

   V5 tag

   Mutation

   C53S, C56S, C397S, C400S

   Promoter

   pCMV

   Available Since

   Aug. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2-CAG-mGreenLantern-KASH

   Plasmid

   #191094

   Purpose

   To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Pantelis Tsoulfas, Murray Blackmore

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   mGreenLantern-KASH
   

   Use

   AAV and Synthetic Biology

   Tags

   mGreenLantern fused to the KASH domain of Nesprin…

   Expression

   Mammalian

   Mutation

   Optimizzation was done using the Human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1A-LivePAR(Y107A)-Hygro

   Plasmid

   #176073

   Purpose

   EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   LivePAR (Y107A)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutation to convert Try107 to Ala

   Promoter

   EF1A

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6_YAP1-V5

   Plasmid

   #173002

   Purpose

   constitutive expression of V5-tagged YAP1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   YAP1-V5 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO-puro-tetON-shOGDH 2497

   Plasmid

   #110942

   Purpose

   Tet-inducible shRNA targeting OGDH

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   shOGDH (OGDH Human)
   

   Use

   Lentiviral

   Promoter

   H1/TO

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV7.1_3xFLAG-WWC1

   Plasmid

   #172994

   Purpose

   constitutive expression of FLAG-tagged WWC1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-WWC1 (WWC1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO-puro-tetON-shOGDH 1714

   Plasmid

   #110941

   Purpose

   Tet-inducible shRNA targeting OGDH

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   shOGDH (OGDH Human)
   

   Use

   RNAi

   Promoter

   H1/TO

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-puro-tetON-shOGDH 2956

    Plasmid

    #110943

    Purpose

    Tet-inducible shRNA targeting OGDH

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shOGDH (OGDH Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    H1/TO

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_ER-RE_MLPmin_BC1063-luc2

    Plasmid

    #227109

    Purpose

    Estrogen receptor response element for luciferase and barcode assays; barcode BC1063

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-HA

    Plasmid

    #162109

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to StrepTagII-ProtC-HA
    

    Use

    Lentiviral

    Tags

    StrepTagII-ProtC-HA

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV2-CAGIG-H2B-V5-mScarlet

    Plasmid

    #191093

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    H2B and mScarlet
    

    Use

    AAV

    Tags

    H2B-V5-mScarlet

    Expression

    Mammalian

    Mutation

    The fusion protein was optimized to the Human cod…

    Promoter

    CMV and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR LAT-FFF/Zap70 iLID-Drop

    Plasmid

    #171032

    Purpose

    Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-tagged. LAT has three Tyr-Phe mutations.

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

    Insert

    LAT (LAT Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiV_Neo_SIK3_CR_T221E

    Plasmid

    #108108

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    change guanine 501 to cytosine (silent mutation),…

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV7.1_3xFLAG-STK3

    Plasmid

    #172990

    Purpose

    constitutive expression of FLAG-tagged STK3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-STK3 (STK3 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Bmpr2ΔKinase gRNA resistant

    Plasmid

    #163411

    Purpose

    Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Expression

    Mammalian

    Mutation

    The kinase domain (202-500 aa) is deleted. gRNA-t…

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-RaichuEV-Rac1(T17N)

    Plasmid

    #164902

    Purpose

    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    RaichuEV-Rac1 (RAC1 Human)
    

    Expression

    Mammalian

    Mutation

    T17N mutation in the Rac1 domain which make the s…

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA4TO R99A CoV-2 nsp1 3xFLAG

    Plasmid

    #176061

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-APPL1-R146A/K152A/R154A

    Plasmid

    #59767

    Purpose

    Expresses APPL1 Endosomal Localization Mutant

    Depositor

    Donna Webb

    Article

    Broussard et al Mol Biol Cell. 2012 Apr;23(8):1486-99. d

    Insert

    APPL1 (APPL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R146A/K152A/R154A

    Promoter

    CMV

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only