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pBN216

Plasmid

#167963

Purpose

express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y713E, Y715E, Y717E, Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN217

Plasmid

#167964

Purpose

express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y708E, Y713E, Y715E, Y717E, Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN262

Plasmid

#167967

Purpose

express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y708E, Y713E, Y715E, Y717E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN202

Plasmid

#167950

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y713E, Y715E, Y717E, Y718E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Ad-MKP-3 C293S

Plasmid

#131525

Purpose

Expresses MKP-3 with C293S mutation in mammalian cells

Depositor

Haiyan Xu

Article

Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

Insert

Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Mutation

changed Cysteine 293 to Serine

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT3

Plasmid

#118985

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Expression

Mammalian

Mutation

K2-mutSP-K1

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT7

Plasmid

#118989

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Expression

Mammalian

Mutation

K2-mutSP-mutK1

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

Plasmid

#91935

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc

Plasmid

#91933

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIMCyp (owl monkey)

Plasmid

#79035

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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TU#1805_CRISPR_unc-73_exon2

Plasmid

#82359

Purpose

to create unc-73B null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2

Plasmid

#79380

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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hs-FL-Kek1-myc2

Plasmid

#41170

Depositor

Norbert Perrimon

Article

Ghiglione et al Cell. 1999 Mar 19;96(6):847-56.

Insert

Kek1 (kek1 Fly)

Tags

2X myc

Expression

Insect

Promoter

Hsp70

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
KHBD00684

Plasmid

#39728

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12630 (tio Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pIS53 SERP1 3'UTR mut

Plasmid

#14500

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

SERP1 3'UTR mutant (SERP1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
3575 pSFFV-neo Bad G148A

Plasmid

#8794

Depositor

Stanley Korsmeyer

Article

Zha et al J Biol Chem 1997 Sep 26;272(39):24101-4.

Insert

Bad (Bad Mouse)

Expression

Mammalian

Mutation

G148A

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
3576 pSFFV-neo Bad R149A

Plasmid

#8795

Depositor

Stanley Korsmeyer

Article

Zha et al J Biol Chem 1997 Sep 26;272(39):24101-4.

Insert

Bad (Bad Mouse)

Expression

Mammalian

Mutation

R149A

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
3577 pSFFV-neo Bad L151A

Plasmid

#8796

Depositor

Stanley Korsmeyer

Article

Zha et al J Biol Chem 1997 Sep 26;272(39):24101-4.

Insert

Bad (Bad Mouse)

Expression

Mammalian

Mutation

L151A

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1

Plasmid

#79379

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 1

Use

CRISPR and Lentiviral

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4

Plasmid

#79382

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 4

Use

CRISPR and Lentiviral

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

Enlarge