We narrowed to 4,223 results for: 424

1. GFP-Mfn1-E209A

   Plasmid

   #141155

   Purpose

   Mammalian expression and labeling of mutant mitofusin 1 (E209A) deficient in GTP hydrolysis

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   MFN 1 (MFN1 Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   changed Glutamic acid at position 209 to Alanine …

   Promoter

   CMV

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 Flag Fli1

   Plasmid

   #66976

   Purpose

   Expression plasmid for mouse Fli1; adds Flag tag to the N-terminus of protein

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Fli1 (Fli1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5/FRT/TO CBX1-APEX2

   Plasmid

   #129642

   Purpose

   Express heterochromatin-localized CBX1-APEX2 fusion in mammalian cells

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   CBX1 (CBX1 Human)
   

   Use

   Tags

   1xFLAG and APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-SYN_mCherry-KIX-mCherry

   Plasmid

   #137007

   Purpose

   AAV backbone, mCherry-KIX acceptor for G-CREB

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

   Insert

   KIX domain (Crebbp Rat)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO APEX2-eIF4E1

   Plasmid

   #129644

   Purpose

   Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   EIF4E1 (EIF4E Human)
   

   Use

   Tags

   APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PD-1-miSFIT-1x

   Plasmid

   #124679

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-Mff(1-61)-PP-GST

   Plasmid

   #73042

   Purpose

   Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site

   Depositor

   David Chan

   Article

   Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

   Insert

   Mff(1-61) (Mff Mouse)
   

   Use

   Tags

   GST-tag and PreScission protease site

   Expression

   Bacterial

   Mutation

   Truncation containing amino acids 1-61

   Promoter

   T7

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO-puro FLAG wildtype, catalytic active lipin 1

   Plasmid

   #32010

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   lipin1 (Lpin1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pEBB HA CCDC22

   Plasmid

   #74903

   Purpose

   Express CCDC22 in mammalian cells

   Depositor

   Ezra Burstein

   Article

   Starokadomskyy et al J Clin Invest. 2013 May;123(5):2244-56.

   Insert

   CCDC22 (CCDC22 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha promoter

   Available since

   June 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. TfR-moxGFP-DHFR

    Plasmid

    #173012

    Purpose

    For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    TfR-moxGFP-DHFR
    

    Use

    Tags

    DHFR and moxGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

    Plasmid

    #226350

    Purpose

    Expression of proteasome substrate with tail for molecular ruler

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V15P

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCRISPR-v1-sgGLS_2

    Plasmid

    #163459

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting GLS

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 2 targeting GLS (GLS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a(ICOS)

    Plasmid

    #177903

    Purpose

    Bacterial expression of his10-tagged human ICOS Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    ICOS (aa 162-199 only) (ICOS Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF1712

    Plasmid

    #141869

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    DPRX (DPRX Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. INPP5D-eGFP D673G mutant

    Plasmid

    #161999

    Purpose

    Mammalian expression vector, containing a GFP tagged phosphatase dead version of INPP5D (D673G)

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    INPP5D (INPP5D Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    D673G

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 Flag MFF iso2 WT

    Plasmid

    #74389

    Purpose

    Flag tagged human MFF isoform 2

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pLC-CUL4A WT-P2A-Puro

    Plasmid

    #124304

    Purpose

    Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PD-1-miSFIT-Ce67

    Plasmid

    #124680

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-KIX (586-672)

    Plasmid

    #99336

    Purpose

    Bacterial expression of mouse CBP KIX domain

    Depositor

    Peter Wright

    Article

    Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

    Insert

    CBP KIX (586-672) (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM33_CLTC_Clathrin propeller

    Plasmid

    #178471

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    CLTC_Clathrin propeller (CLTC Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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