We narrowed to 17,082 results for: EMB

1. TC1614

   Plasmid

   #164873

   Purpose

   CURE-X: C to U RNA Editing, with low off-target effects but also lower efficiency

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   hAPOBEC3A inserted into RfxCas13d (APOBEC3A )
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   human apobec Y132D

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-MSP1D1delltaH4

   Plasmid

   #71713

   Purpose

   Membrane scaffold protein (MSP), helix 4 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCer-4aa-VAMP2

   Plasmid

   #53233

   Purpose

   Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Tags

   4aa-linker and mCerulean

   Expression

   Mammalian

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pMM2-hPMCA4b

   Plasmid

   #47759

   Purpose

   mammalian expression of human PMCA4b

   Depositor

   Emanuel Strehler, John Penniston

   Article

   Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

   Insert

   PMCA4b (ATP2B4 Human)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBTK620

   Plasmid

   #110620

   Purpose

   BTK assembled broad-host-range plasmid encoding GFP driven by CP32 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Jan. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pBTK621

   Plasmid

   #110621

   Purpose

   BTK assembled broad-host-range plasmid encoding GFP driven by CP6 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Sept. 21, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pBTK619

   Plasmid

   #110619

   Purpose

   BTK assembled broad-host-range plasmid encoding GFP driven by CP12b promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. Sec61b-HA-mNG21-10

   Plasmid

   #141213

   Purpose

   Membrane Tethered Split Fluorescent Reporter for ER Retragrade

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   Sec61b-HA-mNG21-10
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV.U6gLacZ.hSyn.flex.H2B.RFP

   Plasmid

   #170373

   Purpose

   Negative control guide, Cre-inducible plasmid expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   Cre inducible H2B RFP
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mbYFPQS

    Plasmid

    #80742

    Purpose

    membrane-targeted biosensor for monitoring changes in intracellular chloride

    Depositor

    Susan Amara, Susan Ingram

    Article

    Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371

    Insert

    YFPQS
    

    Tags

    palmitoylation signal sequence of neuromodulin

    Expression

    Mammalian

    Mutation

    H148Q (CAC to CAG) and V163S (GTG to AGC).

    Promoter

    CMV

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pMM2-hPMCA1b

    Plasmid

    #47758

    Purpose

    mammalian expression of human PMCA1b

    Depositor

    Emanuel Strehler, John Penniston

    Article

    Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

    Insert

    PMCA1 (ATP2B1 Human)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAV116

    Plasmid

    #63183

    Purpose

    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCEP4-hKCNE1

    Plasmid

    #53050

    Purpose

    Expresses beta subunit for IKs channel in mammalian cells

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

    Insert

    Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2-BiNLS-fYap

    Plasmid

    #176623

    Purpose

    expresses optofYap in zebrafish

    Depositor

    Timothy Saunders

    Article

    Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

    Insert

    Yes associated protein (yap1 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    mCherry

    Promoter

    SP6

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MAC_C_TYRO3

    Plasmid

    #187793

    Purpose

    MAC-tagged gene expression of human TYRO3

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    TYRO3 (TYRO3 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV125

    Plasmid

    #63191

    Purpose

    pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDS2142

    Plasmid

    #184273

    Purpose

    Tet-off genome insertion system

    Depositor

    Dominique Sanglard

    Article

    Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.

    Insert

    CatTA
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pN1-Lck-GCaMP3

    Plasmid

    #26974

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

    Insert

    Lck-GCaMP3
    

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NET29A.Hs pEGFP-N2 (1181)

    Plasmid

    #62040

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET29A (TMEM120A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Splice variant

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMV_AA089

    Plasmid

    #216105

    Purpose

    gRNA expression for Cas12a (guide only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    gRNA expression
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge