We narrowed to 8,979 results for: FIE

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pSprayerHE

Plasmid

#165150

Purpose

Sprayer plasmid for PROBER encoding BASU-GAL4 fusion (High Expression)

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

BASU-GAL4

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP Cep164 (Nigg CW324)

Plasmid

#41149

Depositor

Erich Nigg

Article

Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

Insert

Cep164 (CEP164 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pBRC901

Plasmid

#167948

Purpose

express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

Tags

hexa-histidine tag

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 IntS12

Plasmid

#210521

Purpose

Expresses GST-tagged Drosophila IntS12

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS12 (IntS12 Fly)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pX-evoCas9

Plasmid

#107550

Purpose

Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

Depositor

Anna Cereseto

Article

Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

Insert

Humanized S. pyogenes Cas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

M495V/Y515N/K526E/R661Q

Promoter

CBh

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

Plasmid

#62897

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac HT JS-Munc18c

Plasmid

#135555

Purpose

Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

Munc18c

Tags

6xHis tag and TEV site

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70

Plasmid

#62914

Purpose

Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-p65AD-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV human IRS-1 CAAX

Plasmid

#11238

Depositor

Ronald Kahn

Article

Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

Insert

IRS-1 (IRS1 Human)

Tags

CAAX

Expression

Mammalian

Mutation

C-terminal prenylation motif makes IRS-1 localize…

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pSprayer

Plasmid

#165148

Purpose

Sprayer plasmid for PROBER encoding BASU-GAL4 fusion

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

BASU-GAL4

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Burden Monitor lambda version

Plasmid

#66073

Purpose

The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

GFP

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
AAV-CAG-DIO-APEX2NES

Plasmid

#79907

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

APEX2NES

Use

AAV and Cre/Lox

Tags

V5

Expression

Mammalian

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-tdKatushka2-WPRE

Plasmid

#193344

Purpose

AAV-mediated expression of tdKatushka2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

tdKatushka2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-RhoAb-DN

Plasmid

#109580

Purpose

Multisite gateway vector for 3' tagging with dominant negative RhoAb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAb (rhoab Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Venus-BclXL-pEGFP-C1

Plasmid

#177409

Purpose

To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

Depositor

David Andrews

Article

Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

Insert

Bcl-XL

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-P2A-APEX2-VHH[GFP]

Plasmid

#126749

Purpose

Expression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

VHH[EGFP]

Use

Zebrafish muscle expression clone

Tags

mKate2-P2A-APEX2

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-dVP16AD-Hsp70

Plasmid

#62905

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-dVP16AD-Hsp70

Plasmid

#62908

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBITE-EGFRvIII-F265-OKT3

Plasmid

#190679

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F265 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-1P

Plasmid

#202607

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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