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ER-GCaMP6f-AAV

Plasmid

#182501

Purpose

GFAP-ER-GCaMP6f AAV: An AAV5 constructed for ER-GCaMP6f with astrocyte specific GFAP promotor

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Use

AAV

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEIAV-SIN6.1 CBLacZ-LucWm1A

Plasmid

#44167

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Inserts

LacZ
firefly luciferase

Use

Lentiviral and Luciferase

Promoter

CB, CMV enhancer/chicken beta-actin promoter

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pMB1-A-pBAD-dCas9

Plasmid

#190132

Purpose

Level 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ClopHensor-H148G-V224L

Plasmid

#46366

Depositor

Daniele Arosio

Article

Mukhtarov et al Front Mol Neurosci. 2013 Apr 18;6:9. doi

Insert

ClopHensor-H148G-V224L

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP-H148G-V224L is EGFP-T203Y-H148G-V224L

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
pET42a-lambdaN+-L+-GSH

Plasmid

#98894

Purpose

Expresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo method

Depositor

Pascale Legault

Article

Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

Insert

lambdaN+-L+

Tags

GST tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCA_e-2

Plasmid

#202097

Purpose

Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-Ace2_D92N_E199V-mNeon-ST

Plasmid

#129261

Purpose

Express soma-localized Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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MAC-C-GFP

Plasmid

#172973

Purpose

MAC-tagged GFP expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

GFP

Expression

Bacterial and Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pT2-Tbait-UAS-Positron-ST

Plasmid

#129266

Purpose

UAS-driven expression of Positron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

Zebrafish expression

Mutation

D92N, E199V

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.mRuby3

Plasmid

#112179

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Tags

mRuby3

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMB1-A-ptet-dCas9

Plasmid

#190129

Purpose

Level 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxGold

Plasmid

#229948

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mRuby3-ST

Plasmid

#129262

Purpose

Express soma-localized Ace2_D92N_E199V-mRuby3 in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mRuby3-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔRRM1+2

Plasmid

#35267

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 298-429

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
Myc-PSFΔN

Plasmid

#35184

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

N-terminal deletion of residues 9-264

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pMito.iGABASnFR.F102G.mRuby3

Plasmid

#112180

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Tags

mRuby3

Expression

Mammalian

Mutation

F102G

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-PA(WT)-Vav2

Plasmid

#86977

Purpose

PI(DM): Photo-activatable/light sensitive

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/ZF (Vav2 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG5

Plasmid

#193805

Purpose

Protein production of ChemoG5 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG5

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K, T225R; HT7: E143R, E147R, L271E

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-Rabin8

Plasmid

#24910

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

Rabin8 (RAB3IP Human)

Use

Gateway entry vector

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_1

Plasmid

#102963

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_1 (NFS1 Human)

Use

Lentiviral and RNAi

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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