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pZac2.1 hSynapsin1 FLEX NAPA-N SV40

Plasmid

#92283

Purpose

FRET probe; extracellular synapse targeted mCherry, Cre-dependently expressed

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Neuronal

Use

AAV

Promoter

hSynapsin1

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-PKG-hNo.7

Plasmid

#175812

Purpose

The lentiviral vector for PKG #7, a genetically encoded FRET biosensor for monitoring cGMP concentration.

Depositor

Michiyuki Matsuda

Article

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

PKG #7

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-WBP2

Plasmid

#27478

Depositor

Marius Sudol

Article

Chen et al J Biol Chem. 1997 Jul 4. 272(27):17070-7

Insert

WW domain binding protein-2 (WBP2 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
pNP561

Plasmid

#222723

Purpose

Target and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Tags

6xHis

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB701NL3F10H

Plasmid

#141288

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Luciferase; Gateway

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MTK3_036

Plasmid

#123750

Purpose

Encodes mScarlet as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PCDHGC5-EGFP

Plasmid

#119933

Purpose

Protocadherin gamma C5 expression (EGFP-tag at the C-terminus).

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 (Pcdhgc5 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-WNT3A

Plasmid

#108345

Purpose

synchronize trafficking of WNT3A from the ER (RUSH System)

Depositor

David Virshup

Article

Moti et al PLoS One. 2019 Feb 22;14(2):e0212711. do

Insert

WNT3A, Wnt family member 3A (WNT3A Human)

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-3xFLAG-empty-pgk-hph

Plasmid

#48754

Purpose

PiggyBac backbone, CAG promoter, Hygromycin selection, N-terminal 3xFLAG tag

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Promoter

CAG

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only
pLenti6.2-ccdB-Renilla

Plasmid

#87073

Purpose

Lentiviral Gateway-compatible expression vector backbone with a C-terminal Renilla luciferase

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Renilla luciferase

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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Tol2-krt4-RFP

Plasmid

#97149

Purpose

As a control of miRNA sponge

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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mAzami Green-N1 (mAG)

Plasmid

#54798

Purpose

Localization: N1 Cloning Vector, Excitation: 492, Emission: 505

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al J Biol Chem. 2003 Sep 5. 278(36):34167-7

Type

Empty backbone

Tags

mAzami Green

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pJUMP44-2A(sfGFP)

Plasmid

#126988

Purpose

Level 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pspCas9-3'UTR-ST2-com-vector

Plasmid

#136269

Purpose

Expresses SpCas9 and sgRNA Scaffold

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Insert

SpCas9 and sgRNA scaffold

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pG/ER(G)

Plasmid

#108219

Purpose

Yeast expression vector for human estrogen receptor alpha

Depositor

Didier Picard

Article

Liu et al FEMS Microbiol Lett. 1998 Feb 15;159(2):

Insert

Human estrogen receptor a (ESR1 Human)

Expression

Yeast

Promoter

TDH3

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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mPapaya1-N1

Plasmid

#54732

Purpose

Localization: N1 Cloning Vector, Excitation: 530, Emission: 541

Depositor

Robert Campbell, Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Type

Empty backbone

Tags

mPapaya1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pFH102

Plasmid

#128207

Purpose

sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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mTagRFP-pBAD

Plasmid

#54539

Purpose

Localization: Protein Expression Vector , Excitation: 555, Emission: 584

Depositor

Dmitriy Chudakov, Michael Davidson, Dorus Gadella

Article

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Tags

6xHis and mTagRFP

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBbB9a-GFP

Plasmid

#126112

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23113

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGImC_Empty

Plasmid

#92015

Purpose

Empty backbone, includes IRES-mCherry

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Tags

V5

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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