We narrowed to 5,634 results for: pcas

1. pNSU299_YBL055C

   Plasmid

   #166077

   Purpose

   Plasmid for constituive spCas9 and tet-inducible YBL055C targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   YBL055C (near PTC3) (YBL055C Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNSU299_YNR071C

   Plasmid

   #166081

   Purpose

   Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of YNR071C for double stranded break formation in yeast.

   Depositor

   Rebecca Burgess

   Insert

   Promoter of YNR071C (YNR071C Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNSU299_SLA1

   Plasmid

   #166075

   Purpose

   Plasmid for constituive spCas9 and tet-inducible SLA1 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   SLA1 (SLA1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNSU299_PPE1

   Plasmid

   #166083

   Purpose

   Plasmid for constitutive spCas9 and tet-inducible PPE1 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   PPE1 (PPE1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNSU299_PDR12

   Plasmid

   #166093

   Purpose

   Plasmid for constituive spCas9 and tet-inducible PDR12 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   PDR12 (PDR12 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNSU299_TDH2

   Plasmid

   #166082

   Purpose

   Plasmid for constituive spCas9 and tet-inducible TDH2 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   TDH2 (TDH2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNSU299_LYS9

   Plasmid

   #166085

   Purpose

   Plasmid for constituive spCas9 and tet-inducible LYS9 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   LYS9 (LYS9 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX335 Mouse 5' Srcap gRNA B

   Plasmid

   #127905

   Purpose

   Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene

   Depositor

   Mark Groudine

   Insert

   Srcap gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX335 Mouse 5' Srcap gRNA A

   Plasmid

   #127904

   Purpose

   Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene

   Depositor

   Mark Groudine

   Insert

   Srcap gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330 Mouse 3' Ubf gRNA

    Plasmid

    #127902

    Purpose

    WT Cas9 Vector targeting the 3' end of the mouse Ubf gene

    Depositor

    Mark Groudine

    Insert

    gRNA for Mouse 3' Ubf
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C2 G10 png/GFP in casper4

    Plasmid

    #112970

    Purpose

    P element vector for transposon insertion of png-GFP into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Insert

    png-GFP (png Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEM-floxedEGFP-TRI2A

    Plasmid

    #74669

    Purpose

    floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus

    Depositor

    Roberto Giovannoni

    Insert

    floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    pCAGGS

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiCRISPRv2-mCherry

    Plasmid

    #99154

    Purpose

    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

    Depositor

    Agata Smogorzewska

    Inserts

    Cas9
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-ABE8eWQ

    Plasmid

    #161815

    Purpose

    Expresses ABE8eWQ in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPB-US-ECasE

    Plasmid

    #83961

    Purpose

    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.

    Depositor

    Eleanor Chen

    Insert

    ERT2-spCas9-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    please see depositor comments below

    Promoter

    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSaCas9_GFP

    Plasmid

    #64709

    Purpose

    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

    Depositor

    Kiran Musunuru

    Insert

    SaCas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP and 3xFLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNapin-dsRED

    Plasmid

    #127798

    Purpose

    Napin Promoter driven dsRED expression

    Depositor

    Scott Poethig

    Article

    Wu et al Genetics. 2015 May;200(1):35-45. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNapin-eGFP

    Plasmid

    #127797

    Purpose

    Napin Promoter driven eGFP expression

    Depositor

    Scott Poethig

    Article

    Wu et al Genetics. 2015 May;200(1):35-45. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRLG3A

    Plasmid

    #36921

    Depositor

    Manuela Martins-Green

    Article

    Zheng et al Transgenic Res. 2008 Aug;17(4):741-7. Ep

    Inserts

    red fluorescent protein
    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

20. Histone Deacetylase 5

    Plasmid

    #21626

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    Histone Deacetylase 5 (Tcrg-C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

21. hs casper RhoL-N17

    Plasmid

    #17583

    Depositor

    Denise Montell

    Insert

    RhoL (RhoL Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    N17

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

22. hs casper RhoL-V12

    Plasmid

    #17582

    Depositor

    Denise Montell

    Insert

    RhoL (RhoL Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    V12

    Promoter

    Available since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pNSU299_RPL22A

    Plasmid

    #166079

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL22A for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Promoter of RPL22A (RPL22A Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCiB-yin

    Plasmid

    #13845

    Depositor

    Ting Wu

    Article

    Bateman et al Genetics. 2006 Jun . 173(2):769-77.

    Insert

    yellow (y Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCBASceI

    Plasmid

    #26477

    Purpose

    I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site

    Depositor

    Maria Jasin

    Article

    Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

    Insert

    pCBASceI
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSG

    Plasmid

    #169416

    Purpose

    Used for guide cloning

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEF1a-PEmax-P2A-GFP

    Plasmid

    #202000

    Purpose

    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.

    Depositor

    Daniel Geschwind

    Insert

    PEmax-P2A-GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-ABE-P48R

    Plasmid

    #161816

    Purpose

    Expresses ABE-P48R in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTW160C

    Plasmid

    #194801

    Purpose

    PCadBA3 expressing LuxAB

    Depositor

    Tina Wang

    Article

    Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

    Insert

    LuxAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)

    Plasmid

    #160607

    Purpose

    TU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.

    Depositor

    Diego Orzaez

    Insert

    CUP2Gal4AD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge