We narrowed to 8,387 results for: BLI;

1. pHygroMod-Foxd3-3xFlag

   Plasmid

   #37274

   Depositor

   Patricia Labosky

   Insert

   Foxd3 (Foxd3 Mouse)
   

   Use

   Mouse Targeting; Insertion vector for rmce

   Tags

   Flag tag on Foxd3

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2012

   Availability

   Academic Institutions and Nonprofits only

2. TDRD5

   Plasmid

   #32869

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TDRD5 (TDRD5 Human)
   

   Use

   Tags

   His tag (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pAAV-hSyn-TeTxLC-P2A-mTurquoise2

   Plasmid

   #239210

   Purpose

   Expresses tetanus toxin light chain in neurons to inhibit synapse activity

   Depositor

   Erin O'Shea

   Insert

   TeTxLC-P2A-mTurquoise2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBOBI-hPLD3-flag

   Plasmid

   #222640

   Purpose

   lentiviral vector for full length human PLD3 expression (with flag tag)

   Depositor

   David Nemazee

   Insert

   PLD3 (PLD3 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQdCas12a.sggfp(A)

   Plasmid

   #236038

   Purpose

   The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   quorum sensing promoter

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQdCas12a.sggfp(B)

   Plasmid

   #236040

   Purpose

   The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   quorum sensing promoter

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ec dnaN-pHK/P

   Plasmid

   #237239

   Purpose

   over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)

   Depositor

   Michael O'Donnell

   Insert

   dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
   

   Use

   Tags

   his-10+PKA kinase motif+PreScission Protease clea…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRDA_852

   Plasmid

   #185527

   Purpose

   Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Inserts

   First sgRNA cassette
   Second sgRNA cassette
   

   Use

   CRISPR, Cre/Lox, and Lentiviral ; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and Mouse U6

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-tdTomato

   Plasmid

   #232631

   Purpose

   catalytically inactive dCas9 for localization; labeled with tdTomato

   Depositor

   Karsten Rippe

   Insert

   dCas9-tdTomato
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 1996_pCCLsin.PPT.hPGK.EGFP-mCEP83.PRE

    Plasmid

    #231805

    Purpose

    Label the mother centrosome-specific distal appendage protein CEP83 by electroporating an mRNA encoding it as well as an eGFP fusion protein.

    Depositor

    Annette Oxenius

    Insert

    eGFP-mCEP83
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti puro HA-STX3

    Plasmid

    #227590

    Purpose

    Lentiviral plasmid expressing human STX3 protein

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    STX3 (STX3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330-MM002-ZFP143-C-terminal-sgRNA

    Plasmid

    #212706

    Purpose

    Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus

    Depositor

    Elzo de Wit

    Insert

    ZFP143 C-terminal sgRNA (Zfp143 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR/hBIG1

    Plasmid

    #226251

    Purpose

    Entry plasmid of hBIG1 for Gateway system

    Depositor

    Hye-Won Shin

    Insert

    BIG1 (ARFGEF1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR/HA-hBIG2

    Plasmid

    #226252

    Purpose

    Entry plasmid of N-terminally HA-tagged hBIG2 for Gateway system

    Depositor

    Hye-Won Shin

    Insert

    BIG2 (ARFGEF2 Human)
    

    Use

    Entry vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_5xUAS-BFP-WPRE_PGK-HygR-bGHpolyA

    Plasmid

    #216665

    Purpose

    Expresses Gal4 inducible BFP, with hygromycin resistance

    Depositor

    Leonardo Morsut

    Inserts

    BFP
    HygromycinR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti puro HA-VIM

    Plasmid

    #227585

    Purpose

    Lentiviral plasmid expressing human VIM protein

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    VIM (VIM Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)

    Plasmid

    #225432

    Purpose

    Transcriptional unit of BeYDV Rep/RepA (C1:C2)

    Depositor

    Diego Orzaez

    Insert

    pNOS:Rep/RepA:tNOS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEN_TT DNM1L-V5

    Plasmid

    #220359

    Purpose

    Gateway entry vector for an inducible V5-tagged DNM1L

    Depositor

    Kevin Janes

    Insert

    DNM1L (DNM1L Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-UBE3A iso 2 N terminus

    Plasmid

    #185643

    Purpose

    expression of His-tagged UBE3A isoform 2 1-87 in bacterial cells

    Depositor

    Irina Bezsonova

    Insert

    ubiquitin-protein ligase E3A (UBE3A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3.SLC17A2-flag

    Plasmid

    #224419

    Purpose

    Expresses SLC17A2 in mammalian cells

    Depositor

    Jonathan Long

    Insert

    Solute carrier family 17 (sodium phosphate), member 2 (SLC17A2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CD55-Delta3

    Plasmid

    #220872

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-3
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-3, short consensus repeat (SCR) domains 1, …

    Promoter

    CMV

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CD55-Delta4

    Plasmid

    #220873

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-4
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-4, short consensus repeat (SCR) domains 1, …

    Promoter

    CMV

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CD55-Delta1

    Plasmid

    #220870

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-1
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-1, the first short consensus repeat (SCR) d…

    Promoter

    CMV

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CD55-Delta2

    Plasmid

    #220871

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-2
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-2, short consensus repeat (SCR) domains 1 a…

    Promoter

    CMV

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. hs-cre::EBD, UAS-dBrainbow attB

    Plasmid

    #220326

    Purpose

    expresses a heat-shock inducible cre::EBD fusion protein and UAS-dBrainbow

    Depositor

    Erdem Bangi

    Inserts

    Cre::EBD (with intron)
    dBrainbow
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS and hs

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-PE2-P2A-PuroR

    Plasmid

    #196962

    Purpose

    Expresses PE2 with puromycin resistance

    Depositor

    Leopold Parts

    Insert

    PE2-P2A-Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCDF-B-pC

    Plasmid

    #213495

    Purpose

    Encodes Node B of the three-node Turing circuit

    Depositor

    Mark Isalan

    Inserts

    cinI (cinI )
    lacI (lacI )
    lambda cI (lambdap50 )
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    ASV tag and LVA tag

    Expression

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYES2/NTA/Leu-LegA7Δank

    Plasmid

    #216519

    Purpose

    Expression of LegA7 ΔAnk

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔAnk
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 331-454

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYES2/NTA/Leu-LegA7ΔAnk290

    Plasmid

    #216520

    Purpose

    Expression of LegA7 ΔAnk290

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔAnk290
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 290-454

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYES2/NTA/Leu-LegA7ΔNAnk264

    Plasmid

    #216521

    Purpose

    Expression of LegA7 ΔNAnk264

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔNAnk264
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 264-361

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pYES2/NTA/Leu-LegA7ΔNAnk290

    Plasmid

    #216522

    Purpose

    Expression of LegA7 ΔNAnk290

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔNAnk290
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 290-361

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-WT

    Plasmid

    #218834

    Purpose

    Expresses N-terminal BirA*-tagged human wild type TLK1 variant 5 for BioID experiments.

    Depositor

    Travis Stracker

    Insert

    TLK1 (TLK1 Human)
    

    Use

    Tags

    FLAG-BirA*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28-RPL3L-TwStrep

    Plasmid

    #208649

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949

    Depositor

    Quanfu Ma

    Insert

    human RPL3L (ENST00000268661) (RPL3L Human)
    

    Use

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    contain rs753338989, rs746044959, rs752134949, pr…

    Promoter

    T7

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEB018

    Plasmid

    #215568

    Purpose

    Fuses insert of interest to an HA-tag, stop codon and S. commune Hom2 terminator, with flanks directing the construct to S. commune targeted integration locus

    Depositor

    Robin Ohm

    Insert

    3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
    

    Use

    Fungal homologous recombination plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AAV-CAG-K-red-SPOTIT(R271F)

    Plasmid

    #191494

    Purpose

    Red fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

    Depositor

    Wenjing Wang

    Insert

    K-red-SPOTIT(R271F)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    R271F in OR

    Promoter

    CAG

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AAV-CAG-M-red-SPOTIT2(R279F)

    Plasmid

    #191495

    Purpose

    Red fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

    Depositor

    Wenjing Wang

    Insert

    M-red-SPOTIT2(R279F)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    R279F in OR

    Promoter

    CAG

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET50_G495R MDA5

    Plasmid

    #216803

    Purpose

    Expresses a single point mutant of melanoma differentiation-associated protein 5

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 (IFIH1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G495R

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTRIPZ-NUDT17-C-HA-Puro

    Plasmid

    #195182

    Purpose

    Lentiviral vector for DOX-inducible NUDT17 gene expression with C-terminal HA tag. Gateway expression system. Selection by puromycin.

    Depositor

    Stephanie Roessler

    Insert

    NUDT17 (NUDT17 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter and minimal CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDEST26-NAT1-C-HA

    Plasmid

    #195178

    Purpose

    Vector for transient expression of NAT1 gene with C-terminal HA tag, gene flanked by att sites for gateway cloning

    Depositor

    Stephanie Roessler

    Insert

    NAT1 (NAT1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pT7CFE1_polio_3ABC_C147R

    Plasmid

    #212985

    Purpose

    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Poliovirus Type I Mahoney 3ABC C147R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Expression

    Mutation

    cysteine 147 in 3C changed to arginine

    Promoter

    T7

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge