We narrowed to 20,254 results for: ACE;

1. pQLinkHD_EBFP2

   Plasmid

   #118853

   Purpose

   fluorescent protein expression with an N-terminal His-tag

   Depositor

   Marc Birtwistle

   Article

   Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

   Insert

   EBFP2
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   EBFP1.2-I128V/V150I/ D155V/V224R

   Promoter

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL30005

   Plasmid

   #50299

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   N terminal FLAG tag (3x FLAG octapeptide)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pICSL50006

   Plasmid

   #50319

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, luciferase (Photinus pyralis)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pZA2[PTAN:GFP]

   Plasmid

   #60779

   Purpose

   Contains PTAN driving expression of GFP

   Depositor

   Matthew Bennett

   Article

   Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

   Insert

   yemGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Golgi-DKAR

   Plasmid

   #49790

   Purpose

   FRET-based, Golgi-targeted kinase activity reporter for PKD

   Depositor

   Alexandra Newton

   Article

   Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

   Insert

   D kinase activity reporter
   

   Use

   Tags

   CFP, YFP, and eNOS(1-33)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. NAT-TET-OFF -LEU- 4xFLAG

   Plasmid

   #182527

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   7 Copies of TetO Box
   

   Use

   Tags

   4XFLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICH45152

   Plasmid

   #50263

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter, cab1 (AT1g29930, A. thaliana)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBLlSh2

   Plasmid

   #170981

   Purpose

   Expression of ShCAST under control of lacI Plac promoter/repressor pair.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Inserts

   lacI
   ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLlac01

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH71421

   Plasmid

   #51834

   Purpose

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   plant 3'UTRpolyadenylation signal/terminator, Tom H4
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHelper_O

    Plasmid

    #173783

    Purpose

    The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    186 Integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    E59G - please see depositor comments

    Promoter

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHelper_W

    Plasmid

    #173784

    Purpose

    The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    Wphi Integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHelper_D

    Plasmid

    #173781

    Purpose

    The D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    D145 Integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pICH42211

    Plasmid

    #50255

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter nos (A. tumefaciens)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TDGS50

    Plasmid

    #183189

    Purpose

    TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

    Depositor

    Hsiao-Chun Huang

    Article

    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

    Inserts

    DivIVA-GFP(tevR M0050)
    TEV-S219V
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBbB13k-GFP

    Plasmid

    #126122

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23102

    Available Since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUCSh3-mRFP

    Plasmid

    #170983

    Purpose

    Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    mRFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFTK056

    Plasmid

    #171328

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    SpCas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZA2[PLac:mCherry,PTAN:GFP]

    Plasmid

    #60780

    Purpose

    Contains PLac driving expression of mCherry and PTAN driving expression of GFP.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    mCherry
    yemGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-tVYN-Nt∆

    Plasmid

    #154431

    Purpose

    Plasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detection

    Depositor

    Ming Hammond

    Article

    Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

    Insert

    VenusΔC12-NtYcgRΔ-NLucΔ4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFTK088

    Plasmid

    #171360

    Purpose

    AMA1 sequence as Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    AMA1 sequence (short), entry vector providing fungal replication
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge