We narrowed to 3,857 results for: SPL;

1. pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9

   Plasmid

   #107858

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 2) (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CFP-PBD(H83,86L)

   Plasmid

   #11402

   Depositor

   Joel Swanson

   Article

   Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

   Insert

   p21-activated kinase 1 (PAK1 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Obtained with Histidine residues 83 and 86 change…

   Promoter

   Available since

   Feb. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pSicoR-Ef1a-mCh-Puro-Luci

   Plasmid

   #31850

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   Luciferase RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. IBMc326

   Plasmid

   #161949

   Purpose

   Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. tol2-GFAP::CD14

   Plasmid

   #59593

   Purpose

   Magnetic cell sorting of ES cell-derived astrocytes

   Depositor

   Ivo Lieberam

   Article

   Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

   Insert

   human CD14 (CD14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   gfa2

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. GFP-PRMT9 L182A/D183A/I184A/G185A

   Plasmid

   #79676

   Purpose

   mammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

   Depositor

   Steven Clarke

   Article

   Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

   Insert

   PRMT9 (PRMT9 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L182A/D183A/I184A/G185A catalytic mutant

   Promoter

   CMV

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

7. (81) pcDNA3.1-BC2-Nup62-Flag-EPEA

   Plasmid

   #168094

   Purpose

   Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   Nup62-Ubc-Flag-EPEA
   

   Use

   Tags

   BC2-Tag, EPEA-tag, and Flag-tag

   Expression

   Mammalian

   Mutation

   P229A, S283T on Nup62 native sequence

   Promoter

   T7/CMV

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pXPR_sgGFP3

   Plasmid

   #119875

   Purpose

   gRNA expression vector

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   sgGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pB-puro-mU6-CNPshRNA1

   Plasmid

   #126611

   Purpose

   Expresses shRNA against human CNP under mouse U6 promoter

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   CNP ShRNA-1 (CNP Human)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Stat92e pPAcPL

    Plasmid

    #8713

    Depositor

    Jim Darnell

    Article

    Yan et al Cell 1996 Feb 9;84(3):421-30.

    Insert

    Stat92e (Stat92E Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 20, 2005

    Availability

    Academic Institutions and Nonprofits only

11. YFP-Rac1(H40,L61)

    Plasmid

    #11403

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Glutamine 61 mutated to Leucine. Th…

    Promoter

    Available since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pHBS941 TDP43 RRM-GFP VLIMFYW-S

    Plasmid

    #121992

    Purpose

    To test the effect of sequence on TDP43 RRM-GFP droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All hydrophobic residues in IDR1&IDR2 mutated…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHBS1383 TDP43 RRM-GFP FYW-S

    Plasmid

    #118791

    Purpose

    To test the effect of sequence on TDP43 RRM-GFP droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All FYW to S in CTD of TDP43 RRM-GFP reporter

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHBS1386 TDP43 RRM-GFP VLIM-F

    Plasmid

    #118794

    Purpose

    To test the effect of sequence on TDP43 RRM-GFP droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All VLIM to F in CTD of TDP43 RRM-GFP reporter

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHBS1387 TDP43 RRM-GFP KRED-S

    Plasmid

    #118795

    Purpose

    To test the effect of sequence on TDP43 RRM-GFP droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All KRED to S in CTD of TDP43 RRM-GFP reporter

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMYA5T2/pDEST15

    Plasmid

    #98109

    Purpose

    Express CMYA5 (aa 4039 – 4069) Leu allele (4063) with a N-terminal GST tag in bacteria

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    CMYA5 (CMYA5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    rs10043986 (NP_705838.3:p.Pro4063Leu)

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. Cwc25-pRSETA

    Plasmid

    #61043

    Purpose

    Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.

    Depositor

    Nils Walter

    Article

    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

    Insert

    Cwc25
    

    Use

    Tags

    TEV site, Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

18. p177 Dync1i2.C (Ex 1b)

    Plasmid

    #26445

    Depositor

    Elizabeth Fisher

    Article

    Kuta et al PLoS One. 2010 . 5(7):e11682.

    Insert

    cytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1b) (Dync1i2 Mouse)
    

    Use

    Subcloning and sequencing

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    March 24, 2011

    Availability

    Academic Institutions and Nonprofits only

19. p196 Dync1i2.A (Ex 1a)

    Plasmid

    #26446

    Depositor

    Elizabeth Fisher

    Article

    Kuta et al PLoS One. 2010 . 5(7):e11682.

    Insert

    cytoplasmic dynein 1 intermediate chain 2 isoform A (exon 1a) (Dync1i2 Mouse)
    

    Use

    Subcloning and sequencing

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    March 24, 2011

    Availability

    Academic Institutions and Nonprofits only

20. p169 Dync1i1.C

    Plasmid

    #26440

    Depositor

    Elizabeth Fisher

    Article

    Kuta et al PLoS One. 2010 . 5(7):e11682.

    Insert

    cytoplasmic dynein 1 intermediate chain 1 isoform C (Dync1i1 Mouse)
    

    Use

    Subcloning and sequencing

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only