We narrowed to 11,264 results for: VARS

1. pcDNA5/FRT/TO_HaloTag9_SKL

   Plasmid

   #175542

   Purpose

   Peroxysomal expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag9-SKL
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTiger-OptoSOS

   Plasmid

   #86439

   Purpose

   Single-construct blue light optogenetic Ras activator for use in Drosophila

   Depositor

   Jared Toettcher

   Article

   Johnson et al Dev Cell. 2017 Jan 23;40(2):185-192. doi

   Insert

   OptoSOS
   

   Tags

   tagRFP

   Expression

   Insect

   Promoter

   Gal4-UAS

   Available Since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTW211b5

   Plasmid

   #136927

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9(N)-NpuN
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   see manuscript

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-oChIEF-citrine

   Plasmid

   #50974

   Purpose

   AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   ChIEF-citrine
   

   Use

   AAV

   Tags

   citrine

   Mutation

   ChIEF gene is mammalian codon optimized

   Promoter

   human synapsin promoter

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pST-K

   Plasmid

   #44560

   Purpose

   for ectopic constitutive expression of mycobacterial proteins

   Depositor

   Vinay Nandicoori

   Article

   Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

   Type

   Empty backbone

   Use

   E.coli-mycobacteria shuttle vector

   Tags

   Flag tag and His tag

   Expression

   Bacterial

   Promoter

   UV15 promoter

   Available Since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX165-LZ3 Cas9

   Plasmid

   #140561

   Purpose

   Expresses LZ3 Cas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Mutation

   N690C T769I G915M N980K

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pST-KiT

   Plasmid

   #44562

   Purpose

   for integrative inducible expression of mycobacterial proteins

   Depositor

   Vinay Nandicoori

   Article

   Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

   Type

   Empty backbone

   Use

   E.coli-mycobacteria shuttle vector

   Tags

   Flag tag and His tag

   Expression

   Bacterial

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEGFP-30nmSAH-DmrA

   Plasmid

   #61680

   Purpose

   GFP-labeled SAH scaffold with DmrA attachment site

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   mEGFP-30nmSAH-DmrA
   

   Available Since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-oChIEF-tdTomato

   Plasmid

   #50977

   Purpose

   AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   ChIEF-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Mutation

   ChIEF gene is mammalian codon optimized

   Promoter

   human synapsin promoter

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. 5HT2A-APEX2

    Plasmid

    #215558

    Purpose

    Stable, inducible expression of 5HT2A

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    Flag tag-5HT2A(1-452)-APEX2-5HT2A (453-471)
    

    Use

    Lentiviral

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-GluA2-scFv-mNeon-KDEL

    Plasmid

    #230057

    Purpose

    AAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA2 single chain variable fragment (scFv)
    

    Use

    AAV

    Tags

    ER retrieval motif (KDEL) and mNeon

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. ttAtCas12a+int

    Plasmid

    #182384

    Purpose

    Gives very high editing efficiency in barley. Also works in Brassica oleracea

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al bioRxiv 2022.04.28.489853

    Insert

    LbCas12a coding sequence with D156R and Arabidopsis introns
    

    Use

    CRISPR

    Mutation

    D156R + 8 Arabidopsis introns added

    Promoter

    None

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA_e-1

    Plasmid

    #202096

    Purpose

    Receiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pL0R_lacZ

    Plasmid

    #202095

    Purpose

    Receiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCA_o-1

    Plasmid

    #202091

    Purpose

    Receiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

    Plasmid

    #175534

    Purpose

    Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag7-myc_PDGFRtmb
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. hSgk1_v2 Flag

    Plasmid

    #86040

    Purpose

    full length human Sgk1_i2 with Flag tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

    Insert

    Alternate transcript v2 of human Sgk1 (SGK1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    v2

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pD441_WT_hi

    Plasmid

    #128857

    Purpose

    High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Expression

    Bacterial

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a-Core Traptavidin

    Plasmid

    #26054

    Depositor

    Mark Howarth

    Article

    Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

    Insert

    Core Traptavidin
    

    Expression

    Bacterial

    Mutation

    Streptavidin with 2 amino acid mutations: S52G R5…

    Available Since

    Aug. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pET-11-LECT2-I40

    Plasmid

    #183032

    Purpose

    Expression of LECT2 I40 variant protein from T7 promoter

    Depositor

    Gareth Morgan

    Article

    Belonogov et al bioRxiv 2022.03.01.482540

    Insert

    LECT2 (LECT2 Human)
    

    Expression

    Bacterial

    Mutation

    I40 variant

    Promoter

    T7

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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